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Test Code LAB3606 Bilirubin Unconj/Conjugated

Important Note

PROTECT FROM UV LIGHT.

 

If patient is <30 days old, order "NICU Bili."

Additional Codes

NBILI

Clinical System Name

Bilirubin Unconj/Conjugated

Sample Requirements

Specimen:  Whole Blood

Container(s): Lt. Green/Mint Top Lithium Heparin, Lt. Green/Lithium Heparin Microtainer, Gold SST, Gold Microtainer, Dark Green/Sodium Heparin, Red

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL

 

Note: Avoid hemolysis. Protect sample from light. In-patients: wrap paper towel around tube and seal with one piece of tape. Off-site: wrap tube in foil. Specimen stable under fluorescent lighting for up to 4 hours. Method is affected by exposure to UV light, gross hemolysis, and lipemia (very slight lipemia OK).          

          

Processing Instructions

Reject due to: Marked hemolysis, gross lipemia, prolonged light/UV exposure, age of specimen

Spin: Y

Aliquot: Y

Storage location:  CORE 5 Refrigerator

 

Off-site collection: Avoid hemolysis. Protect sample from light by wrapping tube in foil. Specimen should be centrifuged within 2 hours of collection. Spin blood and transfer serum or plasma to plastic tube and refrigerate or freeze. Specimen stable under fluorescent lighting up to 4 hours.

OBCC: For neonates where only a single microtainer (with gel separator) can be collected, spin the blood but do not aliquot. Send in the original collection container. 

Stability

Specimen Type Temperature Time
Plasma or Serum RT

≤ 4 h
Plasma or Serum

2-8 C

≤ 7 d
Plasma or Serum

-20 C or -70 C

≤ 180 d

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Core Lab Chemistry

Phone Number: 206-987-2617 (Client Services)

Synonyms

LAB3606

Bili

Neonatal bilirubin

NBILI

NICU Bili

Availability

STAT Performed TAT
Y 24/7 1 h

Methodology

Method: Colorimetric by Vitros 4600

Analytical Volume:  0.03 mL + 0.03 mL dead space

Reference Range

 

Unconjugated (BU)

Conjugated (BC)

 Sum (BU+BC)

Neonate (0 - 1 month)

0.6 - 10.5 mg/dL

0.0 - 0.6 mg/dL

 0.6 - 11.1 mg/dL

1 month - adult

0.0 - 1.1 mg/dL

0.0 - 0.3 mg/dL

 0.0 - 1.4 mg/dL

Critical level for neonates up to one month

≥ 20.0 mg/dL

≥ 20.0 mg/dL

≥ 20.0 mg/dL

CPT Codes

82248

84311

Critical Values

0-1 M

≥ 20.0 mg/dL