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Test Code LAB3645 SARS-CoV-2 (COVID-19) Qualitative PCR

Clinical System Name

COVID-19 Qualitative PCR









Wuhan Coronavirus


Sample Requirements

Specimen: Nasopharyngeal (NP), mid-turbinate (midT), or oral pharyngeal swab (OP), anterior nasal swab (AN)**


**Only asymptomatic patients should use anterior nasal collections for SARS-CoV-2. Symptomatic patients should still have middle turbinate (MT) or nasopharyngeal (NP) swabs collected.


Container(s): Swab(s) in Universal Transport Media UTM


Other Acceptable Specimen Types: ETT/sputum, BAL, CSF, plasma, and stool.


Please call Central Supply for UTM at ext. 7-2036. Nasophryngeal swabs are available on Demand Flow or through Central Supply.


Collection: Please consult Photo Swab Guide to determine which swab to collect.

Processing Instructions

Reject due to:

Spin: N

Aliquot: N

Temp: 2 - 4 C

Storage Location: CPA 1 Refrigerator, Send Outs rack.


Off-site Collection: Refrigerate specimens for up to 3 days. If holding specimens more than 3 days, freeze at -70 C and transport frozen to the Main Lab.


Specimen Type Temperature Time
All Specimen Types Room Temp 4 h
  Refrigerated 3 d
  Frozen (-70 C) >3 d

Performing Laboratory

University of Washington

Department of Laboratory Medicine

Eastlake Virology

1616 Eastlake Ave E, Suite 320
Seattle, WA 98102


Phone Number: (206) 520-4600


Department: Send Outs

Phone Number: (206) 987-2563



SARS-CoV-2 is detected by Real-time RT PCR using one of the following assays:


The UW SARS-CoV-2 Real-time RT-PCR assay targets two distinct regions within the N gene of SARS-CoV-2 (the causative agent for COVID-19). Amplification of both targets results in a presumptive positive (detectable) test result, while amplification of one of two targets results in an inconclusive result, and amplification of neither target results a negative (non-detectable) test result. Testing is limited to the high complexity CLIA clinical laboratory at UW Virology in Seattle, WA.


The Hologic Emergency Use Authorization (EUA) SARS-CoV-2 Real-time RT-PCR assay targets two conserved regions of the of SARS-CoV-2 (the causative agent for COVID-19) ORF1ab gene. The two regions are not differentiated; amplification of either or both regions is a presumptive positive (detectable) test result and amplification of neither target results a negative (non-detectable) test result.


Analytical Volume: 1 Nasopharyngeal (NP) or Nasal Swab or 0.5 mL for other acceptable specimen types.

Send Out Instructions

Reference Test Name:

SARS-CoV-2 (COVID-19) Qualitative PCR

Reference Number: NCVQLT
Instructions: Send out M - F with the UW courier. Weekends and holidays, send via Delivery Express to UW Eastlake Virology-COVID.