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HelpTest Code LAB3701 Lupus Inhibitor Assay
Clinical System Name
Lupus Inhibitor Assay
Description
Lupus anticoagulants (LA) are immunoglobulins that generally interfere with phospholipid-dependent in vitro coagulation tests.LA is often seen in persons with no underlying diseases and no history of abnormal bleeding. LA also occurs in patients with autoimmune disease or as a reaction to infections or drugs. LA is also associated with thrombosis, including deep venous thrombosis (DVT) and pulmonary emboli (PE), arterial occlusion, pregnancy loss, and microvascular thrombi.
Sample Requirements
Specimen: Whole Blood
Container(s): Lt Blue/Citrate
Required Vol: (1) 2.7 mL
Minimum Vol: (1) 1.8 mL
Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended. Please review the Coagulation Lab Collection Job Aid for detailed instructions.
Processing Instructions: Main Campus
Deliver whole blood to Coag bench. Coag Technologist will process.
Reject due to: clotted, insufficient quantity (underfill), improper collection (overfill), or if patient is on any anticoagulants.
Spin: Y
Aliquot: Y
Storage location: Core 14 Freezer ( -70 C).
Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Processing Instructions: Offsite & Regional Clinics
Reject due to: clotted, insufficient quantity (underfill), improper collection (overfill), or if patient is on any anticoagulants.
Spin: Y
Aliquot: Y
Storage location: -70 C (preferred) or -20 C.
Specimen should be centrifuged within one hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large sample label. Freeze one aliquot of plasma at -70 C (preferred) or -20 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Non-Children's Hospital Offsite collection: Ship completely frozen on dry ice.
Children's Hospital Regional Clinic collection: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Citrated platelet-poor plasma | Room temp | ≤4 h |
| Refrigerated | N | |
| Frozen -20 C or -70 C | ≤3 m |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Coagulation
Phone Number: 206 987-2617 (Client Services)
Synonyms
LAB316
LUPUS
Lupus anticoagulant
Lupus inhibitor
Lupus-like Inhibitor Assay
Staclot-LA
Availability
| STAT | Performed | TAT |
|---|---|---|
| N |
THURSDAY |
1 w |
Methodology
Method: Electromagnetic mechanical clot detection assay by STA-R MAX
Analytical Volume: 600 uL plasma
Limitations: Method is affected by insufficient quantity (underfill), improper collection (overfill), improper processing, and improper storage. High hematocrit greater than or equal to 56% requires a citrate adjusted tube.
Patient should not be on any anticoagulants as this may affect test results.
Reference Range
Negative
CPT Codes
85613
Critical Values
Not defined for this test.