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HelpTest Code LAB3701 Lupus Inhibitor Assay
Clinical System Name
Lupus Inhibitor Assay
Synonyms
LAB316
LUPUS
Lupus anticoagulant
Lupus inhibitor
Lupus-like Inhibitor Assay
Staclot-LA
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Blue/Citrate
Preferred Vol: (1) 2.7 mL
Minimum Vol: (1) 1.8 mL
Note: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended.
If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of a blood transfer device to ensure proper fill.
Specimen should be centrifuged within one hour of collection.
Processing Instructions
Main Campus CPA: Core Technologist will process specimen. Deliver blood directly to Core Coag bench.
Reject due to: Clotted, Insufficient quantity (underfill), or Improper collection (overfill).
Spin: Y
Aliquot: Y
Storage location: Core 14 Freezer ( -70 C).
Core Technologist: Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.
Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Non-Children's Hospital Off-site collection: Ship completely frozen on dry ice.
Children's Regional Clinics: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Citrated platelet-poor plasma | Room temp | ≤4 h |
| Refrigerated | N | |
| Frozen -20 C or -70 C | ≤3 m |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N |
THURSDAY |
1 w |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Coagulation
Phone Number: 206 987-2617 (Client Services)
CPT Codes
85613
Methodology
Method: Electromagnetic mechanical clot detection system by STA-R MAX
Analytical Volume: 0.6 mL plasma.
Limitations: Patient should not be on anticoagulants as this may affect test results.
Method is affected by improper collection, processing, and storage.
High hematocrit greater than or equal to 56% requires a citrate adjusted tube.
Reference Range
Negative
Description
Lupus anticoagulants (LA) are immunoglobulins that generally interfere with phospholipid-dependent in vitro coagulation tests.LA is often seen in persons with no underlying diseases and no history of abnormal bleeding. LA also occurs in patients with autoimmune disease or as a reaction to infections or drugs. LA is also associated with thrombosis, including deep venous thrombosis (DVT) and pulmonary emboli (PE), arterial occlusion, pregnancy loss, and microvascular thrombi.
Critical Values
Not defined for this test.