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Test Code LAB3720 ONC CBC DIFF

Important Note

This order is specific to oncology patients only. Manual differential and smear review is not performed. nRBC count is not included. 

Add on for manual differentials or smear review is not permitted. 

Clinical System Name

Oncology Automated CBC DIFF

Description

This test includes WBC, RBC, HGB, HCT, MCV, MCH, MCHC, RDW, PLT, PMV, automated white blood cell differential, and Absolute Neutrophil Count (ANC).

Synonyms

ONC CBC Diff

LAB3720

Sample Requirements

Specimen: Whole Blood

Container(s): Lavender/EDTA

Preferred Vol: 2.0 - 4.0 mL

Minimum volume: 0.5 mL Microtainer, 1.0 mL Macrotainer

Availability

STAT Performed TAT
Y 24/7 1 h

Stability

Specimen Type Temperature Time
Whole blood RT

≤ 24 h

Whole blood 2-8 C

≤ 48 h

Whole blood -20 C or -70 C

N

Processing Instructions

Reject due to: Insufficient quantity, wrong sample collection, clotted cample, Age of specimen.

Spin: N

Aliquot: N

Temp: RT

Storage location: Core 5 Fridge

 

Offsite collection: Store whole blood at room temperature and transport to Children's Lab immediately. Samples >24 hours old may be rejected. 

Performing Laboratory

Seattle Children's Laboratory

Department

Department: Core Hematology

Phone Number: 206-987-2617 (Client Services)

Methodology

Method:  Sysmex XN-3000, XN-1000, & XN-350: RF/DC detection method, Flow cytometry with semiconductive laser, photometrically, SLS-hemoglobin method, hydrodynamic focusing

Analytical Volume:  0.25 mL  whole blood

CPT Codes

85025

Reference Range

Please see CBC for Red cell indices reference ranges.

Note: No automated differential is reported when WBC <200/CMM

 

Test

Age Range (Male & Female)

    %

   /CMM

Polys

0 - 1 Day

33 - 70   

3000 - 12000

Polys

1 Day - 1 Week

15 - 50

2000 - 6000

Polys

1 Week - 1 Month

15 - 45

1500 - 5000

Polys

1 Month - 1 Year

15 - 45

1500 - 5000

Polys

1 - 4 Years

15 - 70

1500 - 7500

Polys

4 - 10 Years

25 - 70

1800 - 7000

Polys

10 - 14 Years

30 - 70

1500 - 7000

Polys

14 Years - Adult

40 - 75

1500 - 7000

Bands

0 - 24 Hrs

0 - 9

0 - 1500

Bands

1 Day - 1 Week

0 - 9

0 - 1200

Bands

1 Week - 1 Month

0 - 9

0 - 500

Bands

1 Month - 1 Year

0 - 9

0 - 350

Bands

1 - 4 Years

0 - 9

0 - 270

Bands

4 - 10 Years

0 - 9

0 - 240

Bands

10 Years - Adult

0 - 9

0 - 200

Lymphocytes

0 - 1 Day

10 - 35

2000 - 11000

Lymphocytes

1 Day - 1 Week

15 - 70

2000 - 7000

Lymphocytes

1 Week - 4 Years

30 - 70

3000 - 7000

Lymphocytes

4 - 6 Years

20 - 70

1500 - 8500

Lymphocytes

6 - 10 Years

20 - 50

1500 - 5000

Lymphocytes

10 Years - Adult

20 - 40

1100 - 4500

Monocytes

0 Days - 1 Month

0 - 10

0 - 1100

Monocytes

1 Month - Adult

0 - 10

0 - 900

Eosinophils

0 - 24 Hrs

0 - 4

0 - 400

Eosinophils

1 Day - 1 Week

0 - 4

0 - 500

Eosinophils

1 Week - 1 Year

0 - 4

0 - 300

Eosinophils

1 - 10 Years

0 - 4

0 - 250

Eosinophils

10 Years - Adult

0 - 4

0 - 200

Basophils

0 - 1 Week

0 - 1

0 - 100

Basophils

1 Week - 4 Years

0 - 1

0 - 50

Basophils

4 Years - Adult

0 - 1

0 - 40

Immature Granulocytes 0 - Adult 0 - 0.7 0 - 50

Critical Values

White Blood Cell Count (WBC) <1,000/CMM or >50,000/CMM
Hemoglobin (HGB) < 6.0 g/dL
Platelet Count (PLT) < 30,000/CMM or >1,000,000/CMM
Absolute Neutrophil Count (ANC) < 500/CMM