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HelpTest Code LAB3720 ONC CBC DIFF
Clinical System Name
Oncology Automated CBC DIFF
Description
This test includes WBC, RBC, HGB, HCT, MCV, MCH, MCHC, RDW, PLT, PMV, automated white blood cell differential, and Absolute Neutrophil Count (ANC).
Synonyms
ONC CBC Diff
LAB3720
Sample Requirements
Specimen: Whole Blood
Container(s): Lavender/EDTA
Preferred Vol: 2.0 - 4.0 mL
Minimum volume: 0.5 mL Microtainer, 1.0 mL Macrotainer
Availability
| STAT | Performed | TAT |
|---|---|---|
| Y | 24/7 | 1 h |
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole blood | RT |
≤ 24 h |
| Whole blood | 2-8 C |
≤ 48 h |
| Whole blood | -20 C or -70 C |
N |
Processing Instructions
Reject due to: Insufficient quantity, wrong sample collection, clotted cample, Age of specimen.
Spin: N
Aliquot: N
Temp: RT
Storage location: Core 5 Fridge
Offsite collection: Store whole blood at room temperature and transport to Children's Lab immediately. Samples >24 hours old may be rejected.
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Core Hematology
Phone Number: 206-987-2617 (Client Services)
Methodology
Method: Sysmex XN-3000, XN-1000, & XN-350: RF/DC detection method, Flow cytometry with semiconductive laser, photometrically, SLS-hemoglobin method, hydrodynamic focusing
Analytical Volume: 0.25 mL whole blood
CPT Codes
85025
Reference Range
As of 11/18/2025, Polymorphonuclear (Polys) and Band cell populations are no longer reported as distinct cell types. The Neutrophil Count % and the Absolute Neutrophil Count include both polymorphonuclear and band cells.
Please see CBC for Red cell indices reference ranges.
Note: No automated differential is reported when WBC <200/CMM
| Test | Age Range (Male & Female) | % | /CMM |
|---|---|---|---|
| Neutrophil Count % | 0 - 1 Day | 33 - 70 | 3000 - 12000 |
| Neutrophil Count % | 1 Day - 1 Week | 15 - 50 | 2000 - 6000 |
| Neutrophil Count % | 1 Week - 1 Month | 15 - 45 | 1500 - 5000 |
| Neutrophil Count % | 1 Month - 1 Year | 15 - 45 | 1500 - 5000 |
| Neutrophil Count % | 1 - 4 Years | 15 - 70 | 1500 - 7500 |
| Neutrophil Count % | 4 - 10 Years | 25 - 70 | 1800 - 7000 |
| Neutrophil Count % | 10 - 14 Years | 30 - 70 | 1500 - 7000 |
| Neutrophil Count % | 14 Years - Adult | 40 - 75 | 1500 - 7000 |
| Lymphocyte Count % | 0 - 1 Day | 10 - 35 | 2000 - 11000 |
| Lymphocyte Count % | 1 Day - 1 Week | 15 - 70 | 2000 - 7000 |
| Lymphocyte Count % | 1 Week - 4 Years | 30 - 70 | 3000 - 7000 |
| Lymphocyte Count % | 4 - 6 Years | 20 - 70 | 1500 - 8500 |
| Lymphocyte Count % | 6 - 10 Years | 20 - 50 | 1500 - 5000 |
| Lymphocyte Count % | 10 Years - Adult | 20 - 40 | 1100 - 4500 |
| Monocytes Count % | 0 Days - 1 Month | 0 - 10 | 0 - 1100 |
| Monocytes Count % | 1 Month - Adult | 0 - 10 | 0 - 900 |
| Eosinophil Count % | 0 - 24 Hrs | 0 - 4 | 0 - 400 |
| Eosinophil Count % | 1 Day - 1 Week | 0 - 4 | 0 - 500 |
| Eosinophil Count % | 1 Week - 1 Year | 0 - 4 | 0 - 300 |
| Eosinophil Count % | 1 - 10 Years | 0 - 4 | 0 - 250 |
| Eosinophil Count % | 10 Years - Adult | 0 - 4 | 0 - 200 |
| Basophils Count % | 0 - 1 Week | 0 - 1 | 0 - 100 |
| Basophils Count % | 1 Week - 4 Years | 0 - 1 | 0 - 50 |
| Basophils Count % | 4 Years - Adult | 0 - 1 | 0 - 40 |
| Immature Granulocytes % | 0 - Adult | 0 - 0.7 | 0 - 50 |
Critical Values
| White Blood Cell Count (WBC) | <1,000/CMM or >50,000/CMM |
| Hemoglobin (HGB) | < 6.0 g/dL |
| Platelet Count (PLT) | < 30,000/CMM or >1,000,000/CMM |
| Absolute Neutrophil Count (ANC) | < 500/CMM |