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Test Code LAB3724 Anti-Xa for Apixaban Level

Description

The ability of patient plasma to inhibit a chromogenic assay for activated factor X is measured. The apixaban concentration is determined by comparing the inhibition of factor Xa by patient plasma to a calibration curve generated using known concentrations of apixaban.

Clinical System Name

Anti-Xa for Apixaban Level

Synonyms

Anti 10A; Anti Factor X; AntiX; Apixan; Eliquis; Factor 10A; anti factor 10; anti-factor 10; anti-factor X; antiXa

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (3) 1.8 mL or (1) 2.7 mL

Minimum Vol: (2) 1.8 mL

 

Note: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended.

 

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill. 

 

Specimen should be centrifuged within ONE hour of collection.

Processing Instructions

Reject due to: Clotted, hemolyzed, insufficient quantity, or improper collection.

Spin: Y

Aliquot: Y

Temp: -70 C

Storage Location: Core 6 Freezer (-70 C) Send Outs rack.

 

Note: Deliver blood to Core Coag bench. Coag Technologist will process. Specimen should be centrifuged within ONE hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube and make two aliquots of 0.6 mL, affix large Cerner label and store in -70 freezer, Send Out rack.

 

Off-site Collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.

 

Specimen should be centrifuged within ONE hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label. Freeze 2 aliquots of 0.6 mL plasma in plastic tubes at -70C. Ship frozen on dry ice.

Stability

Specimen Type Temperature Time
Citrated Platelet-Poor Plasma Room Temp N
  Refrigerated N
  Frozen Y

 

Availability

STAT Performed TAT
Y, w/Approval Daily 1 d

 

Performing Laboratory

University of Washington Medical Center

Clinical Coagulation Lab
1959 NE Pacific Street
Room NW220
Seattle, WA 98195


Phone Number: (206) 520-4600

Department

Department: Send Outs

Phone Number: (206) 987-2563

CPT Codes

80299

Reference Ranges

See individual components.

Methodology

Method: Chromogenic

Analytical Volume: 0.5 mL Citrate Plasma

Limitations:

Send Out Instructions

Reference Test Name: Anti-Xa for Apixaban Level
Reference Test Code: APIXN1
Instructions: Send out Monday through Friday with the UW courier.