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Test Code LAB3742 Respiratory Virus PCR Panel

Important Note

This test requires a previous positive result on an in-house Rapid Upper Respiratory Viral/Bacterial PCR Panel (LAB3736).

Additional Codes

REVSQF

Clinical System Name

Respiratory Virus PCR Panel

Description

The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the modified FDA approved Panther Fusion® assay for the detection and identification of multiple respiratory viral pathogens.

 

The following viruses are targeted (gene):

  • Adenovirus, AdV (Hexon)
  • human Metapneumovirus, hMPV (nucleocapsid)
  • Rhinovirus, RV (5” UTR)
  • Influenza A, FluA (matrix)
  • Influenza B, FluB (matrix)
  • Respiratory Syncytial Virus, RSV (matrix)
  • Human Parainfuenza virus type 1, hPIV1 (hemagglutinin neuraminidase)
  • Human Parainfuenza virus type 2, hPIV2 (hemagglutinin neuraminidase)
  • Human Parainfuenza virus type 4, hPIV3 (hemagglutinin neuraminidase)
  • Human Parainfuenza virus type 4, hPIV4 (hemagglutinin neuraminidase)
  • Coronavirus (CoV) subtypes OC43 (ORF1ab)
  • Coronavirus (CoV) subtypes HKU1 (ORF1ab)
  • Coronavirus (CoV) subtypes NL63 (Spike)
  • Coronavirus (CoV) subtypes 229R (ORF2ab)

Sample Requirements

Specimen: Aspirate, Bronchial Lavage, Anterior Nares Swab, Nasopharyngeal (NP) Swab, Oropharyngeal (OP) Swab, or Wash

Container(s): Sterile Screw-Capped Container or Universal Transport Media (UTM)

Preferred Vol: 3.0 mL

Minimum Vol: 0.75 mL

 

Note: Acceptable sample types include: BAL, Nasal Wash, or NP swab. NP sample should be collected using a mid turbinate flocked swab, promptly placed in Universal Transport Media (UTM).

Processing Instructions

Reject due to: 

Spin: N

Aliquot: N

Temp: 2 - 8 C

Storage Location: 

  • Monday - Friday: Place sample in the CPA refrigerator, Send Outs rack.
  • Saturday, Sunday, and Holidays: Close batch in MayoACCESS and send via Delivery Express to Eastlake Virology.
    • Note: Call on holidays to confirm that the lab is open to accept samples.

 

Off-site Collection: Refrigerate specimen within four hours of collection. Transport refrigerated.
 

Stability

Specimen Type Temperature Time
  Room Temp

<4 h
  Refrigerated 72 h
  Frozen

Indefinite

 

Performing Laboratory

University of Washington

Department of Laboratory Medicine

Eastlake Virology Lab

1616 Eastlake Ave East, Ste 320

Seattle, WA 98102

 

Phone Number: (206) 520-4600

Department

Department: Send Outs

Phone Number: (206) 987-2563

Synonyms

Adenovirus, AdV (Hexon); Human Metapneumovirus, hMPV (nucleocapsid); Rhinovirus, RV (5” UTR); Influenza A, FluA (matrix); Influenza B, FluB (matrix); Respiratory Syncytial Virus, RSV (matrix); Human Parainfuenza virus type 1, hPIV1 (hemagglutinin neuraminidase); Human Parainfuenza virus type 2, hPIV2 (hemagglutinin neuraminidase); Human Parainfuenza virus type 4, hPIV3 (hemagglutinin neuraminidase); Human Parainfuenza virus type 4, hPIV4 (hemagglutinin neuraminidase); Coronavirus (CoV) subtypes OC43 (ORF1ab); Coronavirus (CoV) subtypes HKU1 (ORF1ab); Coronavirus (CoV) subtypes NL63 (Spike); Coronavirus (CoV) subtypes 229R (ORF2ab)

Availability

STAT Performed TAT
Y M - F: 0700 - 2100, Sa: 0900 - 1600 1 - 2 d

Note: This test is available STAT with UW Lab Med Resident (LMR) approval.

 

Methodology

Method: Real-Time Polymerase Chain Reaction

Analytical Volume: 0.75 mL Aspirate, Bronchial Lavage, or Wash

Limitations:

Reference Range

See individual components

CPT Codes

87633

Send Out Instructions

Reference Lab Test Name:

Respiratory Virus PCR Panel
Reference Lab Test Code:

REVSQF
Instructions:

Send out daily with the UW courier.