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Test Code LAB3742 Respiratory Virus PCR Panel

Important Note

This test requires a previous positive result on an in-house Rapid Upper Respiratory Viral/Bacterial PCR Panel (LAB3736).

Additional Codes

REVSQF

Clinical System Name

Respiratory Virus PCR Panel

Sample Requirements

Specimen: Aspirate, Bronchial Lavage, Anterior Nares Swab, Nasopharyngeal (NP) Swab, Oropharyngeal (OP) Swab, or Wash

Container(s): Sterile Screw-Capped Container or Universal Transport Media (UTM)

Preferred Vol: 3.0 mL

Minimum Vol: 0.75 mL

 

Note: Acceptable sample types include: BAL, Nasal Wash, or NP swab. NP sample should be collected using a mid turbinate flocked swab, promptly placed in Universal Transport Media (UTM).

Processing Instructions

Reject due to: 

Spin: N

Aliquot: N

Temp: 2 - 8 C

Storage Location: 

  • Monday - Friday: Place sample in the CPA refrigerator, Send Outs rack.
  • Saturday, Sunday, and Holidays: Close batch in MayoACCESS and send via Delivery Express to Eastlake Virology.
    • Note: Call on holidays to confirm that the lab is open to accept samples.

 

Off-site Collection: Refrigerate specimen within four hours of collection. Transport refrigerated.
 

Stability

Specimen Type Temperature Time
  Room Temp

<4 h
  Refrigerated 72 h
  Frozen

Indefinite

 

Performing Laboratory

University of Washington

Department of Laboratory Medicine

Eastlake Virology Lab

1616 Eastlake Ave East, Ste 320

Seattle, WA 98102

 

Phone Number: (206) 520-4600

Department

Department: Send Outs

Phone Number: (206) 987-2563

Send Out Instructions

Reference Lab Test Name:

Respiratory Virus PCR Panel
Reference Lab Test Code:

REVSQF
Instructions:

Send out daily with the UW courier.

 

Description

The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the modified FDA approved Panther Fusion® assay for the detection and identification of multiple respiratory viral pathogens.

 

The following viruses are targeted (gene):

  • Adenovirus, AdV (Hexon)
  • human Metapneumovirus, hMPV (nucleocapsid)
  • Rhinovirus, RV (5” UTR)
  • Influenza A, FluA (matrix)
  • Influenza B, FluB (matrix)
  • Respiratory Syncytial Virus, RSV (matrix)
  • Human Parainfuenza virus type 1, hPIV1 (hemagglutinin neuraminidase)
  • Human Parainfuenza virus type 2, hPIV2 (hemagglutinin neuraminidase)
  • Human Parainfuenza virus type 4, hPIV3 (hemagglutinin neuraminidase)
  • Human Parainfuenza virus type 4, hPIV4 (hemagglutinin neuraminidase)
  • Coronavirus (CoV) subtypes OC43 (ORF1ab)
  • Coronavirus (CoV) subtypes HKU1 (ORF1ab)
  • Coronavirus (CoV) subtypes NL63 (Spike)
  • Coronavirus (CoV) subtypes 229R (ORF2ab)

Synonyms

Adenovirus, AdV (Hexon); Human Metapneumovirus, hMPV (nucleocapsid); Rhinovirus, RV (5” UTR); Influenza A, FluA (matrix); Influenza B, FluB (matrix); Respiratory Syncytial Virus, RSV (matrix); Human Parainfuenza virus type 1, hPIV1 (hemagglutinin neuraminidase); Human Parainfuenza virus type 2, hPIV2 (hemagglutinin neuraminidase); Human Parainfuenza virus type 4, hPIV3 (hemagglutinin neuraminidase); Human Parainfuenza virus type 4, hPIV4 (hemagglutinin neuraminidase); Coronavirus (CoV) subtypes OC43 (ORF1ab); Coronavirus (CoV) subtypes HKU1 (ORF1ab); Coronavirus (CoV) subtypes NL63 (Spike); Coronavirus (CoV) subtypes 229R (ORF2ab)

Availability

STAT Performed TAT
Y M - F: 0700 - 2100, Sa: 0900 - 1600 1 - 2 d

Note: This test is available STAT with UW Lab Med Resident (LMR) approval.

 

CPT Codes

87633

Methodology

Method: Real-Time Polymerase Chain Reaction

Analytical Volume: 0.75 mL Aspirate, Bronchial Lavage, or Wash

Limitations:

Reference Range

See individual components