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HelpTest Code LAB3747 Fungal DNA detection by PCR
Clinical System Name
Fungal DNA detection by PCR
Synonyms
28s PCR, 28S sequencing, broad range fungal PCR, broad range fungi PCR, fungal 28S, fungal identification, fungal ITS, Fungal PCR, fungal sequencing, fungi identification, Fungi PCR, fungi sequencing, ITS PCR, ITS sequencing, molecular fungal, molecular fungi, universal fungal PCR, universal fungi PCR
Sample Requirements
Specimen: See Note
Container(s): Sterile Screw-Capped Container
Preferred Vol: 3.0 mL fluid
Minimum Vol: Diagnostic yield generally proportional to specimen size
Note: Acceptable sample types include: tissue (Fresh, Fixed, or paraffin embedded), or fluid from a sterile site. If specimen is fluid, use sterile technique to aliqout 2 mL fluid into sterile screw top container.
Also Accepted: E-swabs and UTM (Universal Transport medium); Formalin-fixed paraffin-embedded tissues (FFPE, PET); non-blood body fluids collected in Vacutainer tubes without preservative; Lavender top (EDTA) tube.
Processing Instructions
Reject due to: Tissues free-floating in formalin; samples containing citrate, or heparin; Blood/Serum specimens; Stool; Sputum; Swab/fluid collected in tube containing agar.
Spin:N
Aliquot: N
Temp:-20 C
Storage location: CPA freezer sendouts rack.
Off-site collection: Transport frozen.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Room Temp |
Unacceptable |
|
| Refrigerated | Unacceptable | |
| Frozen |
Indefinite |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N |
M - F |
5 - 7 d |
Preliminary results in 2 to 4 days.
Performing Laboratory
University of Washington
Dept of Laboratory Medicine
UW Microbiology Molecular Diagnostics
1959 NE Pacific St, NW220
Seattle, WA 98195
Phone Number: (206) 520-4600
Department
Department: Send Outs
Phone Number: (206) 987-2563
CPT Codes
87801
Methodology
Method: DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing
Analytical Volume: 1.0 mL Fluid or 1 Cubic Centimeter Tissue
Limitations:
Reference Range
| Negative |
Send Out Instructions
| Reference Test Name: |
Fungal DNA Detection by PCR |
| Reference Test Number: | |
| Instructions: |
Send out Monday thru Friday with the UW courier. |