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Test Code LAB3749 Mycoplasma and Ureaplasma DNA detection by PCR  

Important Note

This test detects both mycoplasma and ureaplasma DNA.

Clinical System Name

Mycoplasma and Ureaplasma DNA detection by PCR  

Synonyms

Molecular Mycoplasma, Molecular Ureaplasma, Mycoplasma genitalium, Mycoplasma hominis, Mycoplasma identification, Mycoplasma PCR, Mycoplasma pneumoniae, Synonyms: 16S PCR, universal PCR, Ureaplasma identification, Ureaplasma parvum, Ureaplasma PCR, Ureaplasma urealyticum

Sample Requirements

Specimen: CSF, Swab, or Tissue

Container(s): CSF Tube, Sterile Container, or Universal Transport Media (UTM), 

Preferred Vol: 1.0 mL Fluid or 0.5 cc Tissue

Minimum Vol: 1.0 mL Fluid or 0.5 cc Tissue

 

Note: Specimen may be a swab in universal transport media (UTM) or tissue in UTM. Swab or tissue MUST be placed in UTM at time of collection.

 

Acceptable Specimens: E-Swabs and formalin-fixed paraffin embedded (FFPE) tissues.

Processing Instructions

Reject due to: Tissues free-floating in formalin; samples containing SPS, citrate, or heparin; Blood/Serum specimens, stool and extracted DNA.

Spin: N

Aliquot: N

Temp: -20 C

Storage Location: CPA -20 freezer, Send Outs rack.

 

Off-site Collection:

Stability

Specimen Type Temperature Time
CSF, Swab, or Tissue Room Temp Unacceptable (Unless FFPE)
  Refrigerated 2 h
  Frozen Indefinite

 

Availability

STAT Performed TAT
N Daily

5 - 7 d

 

Performing Laboratory

University of Washington Medical Center

Department of Laboratory Medicine

Microbiology (MC)

1959 NE Pacific Street, Room NW120

Seattle, WA 98195

 

Phone Number: (206) 520-4600

Department

Department: Send Outs

Phone Number: (206) 987-2563

Methodology

Method: Real-Time PCR

Analytical Volume: 1.0 mL Fluid or 0.5 cc Tissue

Limitations: Diagnostic yield generally proportional to specimen size.

Send Out Instructions

Reference Lab Test Name:

Mycoplasma and Ureaplasma DNA detection byPCR
Reference Lab Test Code: MPLDNA
Instructions: Send daily with the UW courier.

 

Description

This test can detect Mycoplasma hominis, Mycoplasma genitalium, Mycoplasma pneumoniae, and Ureaplasma species.