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Test Code LAB3768 Nicotine and Metabolites, Random, Urine

Reporting Name

Nicotine and Metabolites, U

Useful For

Monitoring tobacco use

 

Monitoring patients on nicotine-replacement therapy for concurrent use of tobacco products

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Urine


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Container/Tube: Plastic, 5 mL, aliquot tube

Specimen Volume: 3 mL

Collection Instructions:

1. Collect a random urine specimen.

2. No preservative.


Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 28 days
  Frozen  365 days
  Ambient  28 days

Reference Values

Non-tobacco user with no passive exposure:

NICOTINE

<5.0 ng/mL

 

COTININE

<5.0 ng/mL

 

ANABASINE

<2.0 ng/mL

 

NORNICOTINE

<2.0 ng/mL

Day(s) Performed

Monday through Thursday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

80323

G0480 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
NICOU Nicotine and Metabolites, U 43125-4

 

Result ID Test Result Name Result LOINC Value
82510 Nicotine 3854-7
21321 Cotinine 10366-3
21324 Nornicotine 33917-6
21323 Anabasine 33915-0

Method Description

Nicotine and metabolites are extracted from urine by solid-phase extraction techniques. The extract eluate is quantified by high-performance liquid chromatography tandem mass spectrometry.(Moyer TP, Charlson JR, Enger RJ, et al. Simultaneous analysis of nicotine, nicotine metabolites, and tobacco alkaloids in serum or urine by tandem mass spectrometry, with clinically relevant metabolic profiles. Clin Chem. 2002;48[9]:1460-1471; Oh J, Park MS, Chun MR, et al. A simple and high-throughput LC-MS/MS method for simultaneous measurement of nicotine, cotinine, 3-OH cotinine, nornicotine, and anabasine in urine and its application in the general Korean population. J Anal Toxicol. 2022:46[1]:25-36. doi:10.1093/jat/bkaa177)

Report Available

2 to 5 days

Reject Due To

Gross hemolysis OK
Gross icterus OK

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Forms

If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.