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HelpTest Code LAB3816 Mpox Virus Qualitative PCR
Description
The detection of monkeypox virus DNA by real-time PCR amplification. The primers used in this PCR reaction amplify the F3L gene (Maksyutov et al., J Virological Methods 2016) and the probe hybridizes the target sequences between the primers that specifically identifies the PCR amplicon. The minimum virus level that gives a positive result in 95% of replicates is 328 copies/mL in either VTM/UTM or Aptima multitest tubes.
Clinical System Name
Mpox Virus Qualitative PCR
Synonyms
Orthopoxvirus; Monkeypox, MPX; Monkey Pox; Mpox
Sample Requirements
Specimen: Skin Lesion, Rectal, or Genital Swab OR Cerebrospinal Fluid
Container(s): Swab(s) in Universal Transport Media (UTM) OR CSF Tube
Other Acceptable Specimen Types: CSF
Please call Central Supply for UTM at ext. 7-2036. Swabs are available on Demand Flow or through Central Supply.
Processing Instructions
Reject due to:
Spin: N
Aliquot: N
Temp: 2 - 4 C
Storage Location: CPA 1 Refrigerator, Send Outs rack.
Off-site Collection: Refrigerate specimens for up to 7 days. If holding specimens more than 7 days, freeze at -70 C and transport frozen to the Main Lab.
Stability
| Specimen Type | Temperature | Time |
| All Specimen Types | Room Temp | 4 h |
| Refrigerated | 7 d | |
| Frozen (-70 C) | Indefinitely |
Performing Laboratory
University of Washington
Department of Laboratory Medicine
Eastlake Virology
1616 Eastlake Ave E, Suite 320
Seattle, WA 98102
Phone Number: (206) 520-4600
Department
Department: Send Outs
Phone Number: (206) 987-2563
Methodology
Method: Laboratory developed PCR
Analytical Volume: 1 Skin Lesion, Rectal, or Genital Swab or 0.5 mL for Cerebrospinal Fluid.
Send Out Instructions
| Reference Test Name: |
Monkeypox Virus Qualitative PCR |
| Reference Number: | MPXQLT |
| Instructions: | Send out daily with the UW courier. |