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Test Code LAB3825 Cell Free DNA (cfDNA) Molecular Genetics Lab Hold

Important Note

cfDNA Molecular Genetics Lab Hold requests are restricted to clinical lab testing ordered by Seattle Children's Hospital providers only. 

BEFORE ordering cfDNA MGL hold  for collection we strongly suggest that each case is discussed with a  Lab Genetic Counselor. 

cfDNA MGL hold requests are limited to the following circumstances:

  • One specific internal clinical molecular test is planned, AND
  • An alternative specimen collection (e.g. saliva, buccal) is not an option; AND
  • There is a significant hardship to deferral of sample collection until after genetic test preauthorization is obtained.

After sample collection, all requests will be reviewed for approval by the Lab Genetic Counselor team, 206-987-5400 or LabGC@seattlechildrens.org.  Genetic testing must be approved by the Lab Test Stewardship Team. Some samples may be discarded if specified criteria are not met.

Clinical System Name

Cell Free DNA Molecular Genetics Lab Hold

Description

To minimize the risk of excessive financial liability for patient family and the organization, an insurance pre-authorization outcome must be obtained prior to ordering genetic testing (Policy & Procedures: Insurance Pre-Authorization for Genetic Testing).  

 

Best practice is deferral of sample collection until the insurance pre-authorization outcome is determined. Short-term cfDNA storage (maximum 1 year) at SCH Laboratory is available for the rare instances when a sample collection cannot be deferred during the pre-authorization process. This service, cfDNA MGL Hold,  should be used only when an alternative sample collection (saliva, buccal) cannot be utilized for the requested test and it is a signficant hardship for a family to return to a SCH laboratory for sample.  

 

DNA not utilized for testing within one year of the date of collection will be discarded.  cfDNA MGL hold samples are restricted to clinical use only.   

 

Sample Requirements

Sample Requirement Important notes
Blood 16-20 mL whole blood in Streck Cell-Free DNA BCT® tube Sample must be received within 5 days of collection. Tube cannot be frozen.
Lymphatic cyst fluid 1-8 mL in Streck Cell-Free DNA BCT® tube Sample must be received within 5 days of collection. Tube cannot be frozen.

Processing Instructions

Specimen Type Description

Temperature

 Storage instructions
Whole blood Streck tube Room Temp Place in CPA Cytogenetics room temp box 
Lymphatic cyst fluid Streck tube Room Temp Place in CPA Cytogenetics room temp box 

 

Off-site collection: Transport blood or lymphatic cyst fluid at room temperature.

Stability

Specimen Type Temperature Time
Cell-free DNA, whole blood Room Temp 2 weeks
Lymphatic cyst fluid Room Temp 2 weeks

Availability

Performed
Monday - Friday

 

Performing Laboratory

Seattle Children's Laboratory

Department

Department:  Molecular Genetics Laboratory, labdnabank@seattlechildrens.org

Phone: 206-987-3872

Lab Client Services: 206-987-2617 or labclientservices@seattlechildrens.org

Lab Genetic Counselor: 209-987-5400 or LabGC@seattlechildrens.org

Reference Range

A result documenting a sample is being held and the discard date will post to chart.