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HelpTest Code LAB3873 Hepatitis B Core, Total Antibody
Clinical System Name
Hepatitis B Core, Total Antibody
Synonyms
Hep B; Core Total Antibody
Sample Requirements
Specimen: Whole Blood
Container(s): Gold, Gold Microtainer, or Red Top-Plain
Preferred Vol: 4.0 mL
Minimum Vol: 1.0 mL
Processing Instructions
Reject due to: Cord blood, neonatal specimens, cadaver specimens, heat-inactivated specimens, or body fluids such as saliva, urine, amniotic fluid, or pleural fluid. Specimens with obvious microbial contamination or are gross hemolyzed.
Spin: Y
Aliquot: Y
Temp: 2 - 8 C
Storage Location: CPA refrigerator, Send Outs rack.
Off-Site Collection: Centrifuge whole blood, transfer 2.0 mL (Min. 0.5 mL) serum to plastic aliquot container affixed with a large Epic label. Refrigerate serum.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Room Temp | 3 d |
| Refrigerated | 7 d | |
| Frozen | Indefinite |
Note: Avoid more than three freeze/thaw cycles.
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | M-F | 2 - 4 d |
Performing Laboratory
University of Washington
Department of Laboratory Medicine
Eastlake Virology Lab
1616 Eastlake Ave East, Ste 320
Seattle, WA 98102
Phone Number: (206) 520-4600
Department
Department: Send Outs
Phone Number: (206) 987-2563
CPT Codes
86704
Methodology
Method: Chemiluminescence Immunoassay
Analytical Volume: 0.5 mL serum
Limitations:
Reference Range
Reference values accompany patient report.
Description
The Qualitative detection of Hepatitis B virus Core IgG and IgM antibodies (HBcAb) in human sera using the FDA approved Abbott ARCHITECT CORE test two-step chemiluminescent immunoassay.
In the first step, sample, assay diluent, and Hepatitis Core Antigen (HBcAg) coated paramagnetic microparticles are combined. Anti-HBc antibodies present in the sample binds to the HBcAg coated microparticles. In the second step, anti-human IgG and IgM acridinium-labeled conjugate is added, which binds to IgG and IgM anti-HBc. Then pre-trigger and trigger solutions are added to the reaction mixture. The resulting chemiluminescent reaction is measured as relative light units (RLUs).
The presence or absence of anti-HBc antibodies in the sample is determined by comparing the chemiluminescent signal in the reaction to the cutoff signal determined from an active ARCHITECT CORE calibration. Specimens with signal to cutoff (S/CO) values ≥ 1.00 are considered reactive for HBcAb. Specimens with S/CO values < 1.00 are considered nonreactive.
Send Out Instructions
| Reference Lab Test Name: |
Hepatitis B Core Antibody (HBcAb) |
| Reference Lab Test Code: | |
| Instructions: |
Send out daily with the UW courier. |