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Test Code LAB3878 Histoplasma and Blastomyces Antigen, Enzyme Immunoassay, Urine


Specimen Required


Supplies: Sarstedt Aliquot tube, 5 mL (T914)

Container/Tube: Plastic vial

Specimen Volume: 3 mL

Collection Instructions:

1. Collect a random urine specimen, with no preservative.

2. Do not centrifuge to remove particulates.


Useful For

Diagnosing Histoplasma capsulatum or Blastomyces dermatitidis infection without differentiation between the organisms

 

Monitor antigen levels following initiation of antifungal treatment

Method Name

Enzyme Immunoassay (EIA)

Reporting Name

Histoplasma/Blastomyces Ag, EIA, U
Seattle Children's Hospital Note:

SCH Clinical System Name: Histoplasma and Blastomyces Antigen, Urine

Specimen Type

Urine

Specimen Minimum Volume

2.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 14 days
  Frozen  28 days
  Ambient  7 days

Reject Due To

Gross hemolysis Reject
Turbid
Colored		 Reject

Reference Values

Histoplasma/Blastomyces Antigen Result:

Not Detected

 

Histoplasma/Blastomyces Antigen Value:

Not Detected: 0.0 ng/mL

Detected: <1.3 ng/mL

Detected: 1.3-20.0 ng/mL

Detected: >20.0 ng/mL

 

Reference values apply to all ages.

Method Description

The assay detects Blastomyces dermatitidis antigen in human serum samples using specific, proprietary antibodies in an enzyme-linked immunosorbent assay format. The detection method involves an enzyme/substrate system with the level of urinary B dermatitidis antigen proportional to the assay signal. The patient specimen result is compared to a cutoff calibrator and a standard curve of a series of assay calibrators (1.25 to 20.00 ng/mL) to determine the presence or absence of antigen and, if present, to establish a quantitative level of B dermatitidis serum antigen.(Package insert: Blastomyces dermatitidis Urinary Antigen Detection Kit. Gotham Biotechnology; V1, R3, 06/2021)

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 to 3 days
Seattle Children's Hospital Note:

Expected TAT: 3 - 5 d

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87449

LOINC Code Information

Test ID Test Order Name Order LOINC Value
UHBAG Histoplasma/Blastomyces Ag, EIA, U 105123-4

 

Result ID Test Result Name Result LOINC Value
UHBAR Histoplasma/Blastomyces Ag Result 104871-9
DEXBH Histoplasma/Blastomyces Ag Value 104872-7