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Test Code LAB3916 Constitutional FISH, Amniotic Fluid

Important Note

This test is to be ordered by Fetal Center providers ONLY. Amniotic fluid should be sent directly to UW Clinical Genomics Laboratory upon collection. Please scan Constitutional Test Request Form to referencelabteam@seattlechildrens.org. 

Description

This test can be used to detect aneuploidy of single select chromosomes, microdeletions, microduplications, or polyploidy (with a set of probes) using interphase nuclei and/or metaphase chromosomes.

 

FISH probes designed to detect chromosome number can be used on interphase cells to give a rapid (1-3 days) diagnosis of constitutional conditions caused by changes in whole chromosome copy number (e.g. Down syndrome due to trisomy 21).

 

FISH probes can also be used to diagnose constitutional syndromes caused by chromosome deletions and duplications that are too small to be seen with routine karyotyping. The ordering provider should have a high clinical suspicion for a specific disorder if choosing this type of FISH analysis. Otherwise, a chromosomal microarray analysis may be a better test method to use.

Sample Requirements

Amniotic Fluid:

  • Collect 15-30 mL of fluid obtained under sterile conditions into Corning tissue culture tubes or tubes from a Baxter amniocentesis tray kit. Discard the first 1 mL of fluid or use for af-AFP testing.

Processing Instructions

These specimens are not recieved by SCH Lab - they are sent directly from the Fetal Center to UW CGL. 

Handling instructions for Fetal Center:

  • Maintain and transport samples at ambient temperature unless otherwise indicated in the Collection section.
  • Sample collection kits are available upon request. Kits include sample collection tubes, transport media (when required), a biohazard bag with absorbent material, a test requisition, and FedEx shipping materials. Contact the lab at 206.598.4488 to request a kit.
  • A completed request form must accompany all orders.
  • Specimens are accepted 24 hrs/day, 7 days/week.

Performing Laboratory

Cytogenetics and Genomics Laboratory
University of Washington Medical Center, Room NW-125
1959 NE Pacific Street
Seattle, WA 98195-6100

 

Ph: 206.598.4488

Department

Department: Send Outs

Phone: (206) 987-2563

Synonyms

FISHCONST2; FISH Analysis for Constitutional Abnormalities

Availability

STAT Performed TAT
Y Performed daily   1-2 days