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HelpTest Code LAB48 Amylase Serum
Clinical System Name
Amylase Serum
Synonyms
LAB48
AMYL
Sample Requirements
Specimen: Whole Blood
Container(s): Gold SST, Gold Microtainer, Red
Preferred Vol: 1.0 mL
Minimum Vol: 0.5 mL
Note: Test is affected by lipemia, oxalate, fluoride, citrate, and heparin. Test is not affected by hemolysis and icterus. Do NOT freeze samples.
Processing Instructions
Reject due to: Wrong collection container, frozen specimen, age of specimen
Spin: Y
Aliquot: Y
Storage location: CORE 5 Refrigerator
Off-site collection: Specimen must be serum; Do NOT use green top tubes. Specimen should be centrifuged within 2 hours of collection. Spin blood, and transfer serum to plastic tube and refrigerate. Do NOT freeze samples.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | RT |
≤ 7 d |
| Serum | 2-4 C |
≤ 30 d |
|
-20 C or -70 C |
N |
Availability
| STAT | Performed | TAT |
|---|---|---|
| Y | 24/7 | 1 h |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Core Lab Chemistry
Phone Number: 206-987-2617 (Client Services)
CPT Codes
82150
Methodology
Method: Spectrophotometric, Enzyme Rate by Vitros 4600
Analytical Volume: 0.02 mL + 0.03 mL dead space
Reference Range
| All Ages |
≤ 115 IU/L at 37o C |
Description
Amylase is an amylolytic digestive enzyme produced by the exocrine pancreas and salivary glands. Amylase is increased in acute pancreatitis, pancreatic abscess or pseudocyst, pancreatic trauma, amyloidosis, pancreatic neoplasm, common-bile-duct obstruction, and after thoracic surgery. Increased amylase activity may be found in mumps parotitis and renal insufficiency.