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HelpTest Code LAB492 Protein S Antigen, Free
Clinical System Name
Protein S Antigen, Free
Synonyms
Protein S AG-Function
Protein S Level
Fractionated Protein S
PSAGF
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Blue/Citrate
Preferred Vol: (1) 2.7 mL
Minimum Vol: (1) 1.8 mL
Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended. Please review the Coagulation Lab Collection Job Aid for detailed instructions.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Citrated Platelet-Poor Plasma | Room temp | N |
| Refrigerated | N | |
| Frozen | Y |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | M, Th | 1 - 8 d |
Performing Laboratory
Harborview Medical Center
Clinical Coagulation Lab
325 9th Ave
Room GWH 47
Seattle, WA 98195
Phone Number: (206) 744-3128
Department
Department: Send Outs
Phone Number: (206) 987-2563
Methodology
Method: Optical, Stago STA LIA Free Protein S Antigen Assay
Analytical Volume: 500 uL Citrate Plasma
Limitations: Method is affected by moderate lipemia, moderate hemolysis, moderate icterus, insufficient quantity (underfill), improper collection (overfill), improper processing, and improper storage. High hematocrit greater than or equal to 56% requires a citrate adjusted tube.
Reference Range
| Female | Male | ||
| Age | % Range | Age | % Range |
| 0 - 3 months | 15 - 150 | 0 - 3 months | 15 - 150 |
| 3 - 6 months | 35 - 150 | 3 - 6 months | 35 - 150 |
| 6 mos - 1 y | 47 - 150 | 3 months | 47 - 150 |
| 1 yr - 6 y | 49 - 150 | 1 y - 6 y | 49 - 150 |
| 6 y - 10 y | 58 - 150 | 6 y - 10 y | 58 - 150 |
| 10y - | 55 - 150 | 10y - | 65 - 150 |
Note: Elevated Protein S is not associated with thrombosis or bleeding.
CPT Codes
85306
Processing Instructions: Main Campus
Deliver whole blood to Coag bench. Coag Technologist will process.
Reject due to: clotted, hemolyzed, insufficient quantity (underfill), or improper collection (overfill).
Spin: Y
Aliquot: Y
Temp: -70 C
Storage Location: CPA 2 Freezer ( -70 C) Send Out rack.
Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Send Out Instructions
| Reference Test Name: | Protein S Antigen (Free) |
| Reference Test Number: | PSAGF |
| Instructions: | Send out Monday through Friday with the UW/HMC courier. |
Processing Instructions: Offsite & Regional Clinics
Reject due to: clotted, insufficient quantity (underfill), or improper collection (overfill).
Spin: Y
Aliquot: Y
Storage location: -70 C (preferred) or -20 C.
Specimen should be centrifuged within one hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large sample label. Freeze one aliquot of plasma at -70 C (preferred) or -20 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Non-Children's Hospital Offsite collection: Ship completely frozen on dry ice.
Children's Hospital Regional Clinic collection: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.