Test Code LAB518 Androstenedione, Serum
Additional Codes
ANDROSTEN
Reporting Name
Androstenedione, SAndrostenedione
Useful For
Diagnosis and differential diagnosis of hyperandrogenism, in conjunction with measurements of other sex steroids
Diagnosis of congenital adrenal hyperplasia (CAH), in conjunction with measurement of other androgenic precursors, particularly, 17-alpha-hydroxyprogesterone (OHPG), 17 alpha-hydroxypregnenolone, dehydroepiandrosterone sulfate (DHEA-S), and cortisol
Monitoring CAH treatment, in conjunction with testosterone, OHPG, DHEA-S, and DHEA
Diagnosis of premature adrenarche, in conjunction with measurement of follicle-stimulating hormone and luteinizing hormone as well as other adrenal and gonadal sex-steroids and their precursors
Ordering Guidance
Additional Testing Requirements
For diagnosis and differential diagnosis of hyperandrogenism, an initial workup in adults should also include total and bioavailable testosterone (TTBS / Testosterone, Total and Bioavailable, Serum) measurements. Depending on results, this may be supplemented with measurements of sex hormone-binding globulin (SHBG1 / Sex Hormone-Binding Globulin, Serum) and other androgenic steroids (eg, dehydroepiandrosterone sulfate [DHEA-S]).
For diagnosis of congenital adrenal hyperplasia (CAH), the following assays should also be ordered:
-OHPG / 17-Hydroxyprogesterone, Serum
-DHES1 / Dehydroepiandrosterone Sulfate, Serum
-CORT / Cortisol, Serum
For monitoring CAH treatment, the following assays should also be ordered:
-TTST / Testosterone, Total, Mass Spectrometry, Serum
-OHPG / 17-Hydroxyprogesterone, Serum
-DHES1 / Dehydroepiandrosterone Sulfate, Serum
-DHEA_ / Dehydroepiandrosterone [DHEA], Serum.
For diagnosis of premature adrenarche, the following assays should also be ordered:
-FSH / Follicle-Stimulating Hormone [FSH], Serum
-LH / Luteinizing Hormone [LH], Serum
-TTBS / Testosterone, Total and Bioavailable, Serum or TGRP / Testosterone, Total and Free, Serum
-EEST / Estradiol, Serum
-DHES1 / Dehydroepiandrosterone Sulfate, Serum
-DHEA_ / Dehydroepiandrosterone (DHEA), Serum
-SHBG1 / Sex Hormone-Binding Globulin, Serum
-OHPG / 17-Hydroxyprogesterone, Serum
Specimen Required
Collection Container/Tube: Red top (serum gel/SST are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 0.6 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial
Collect 1.2 mL whole blood in a Red Top.
Specimen Type
Serum RedSpecimen Minimum Volume
0.25 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum Red | Refrigerated (preferred) | 28 days | |
Frozen | 28 days | ||
Ambient | 7 days |
Day(s) Performed
Monday through Friday
TAT: 4 - 7 days
Performing Laboratory
Mayo Clinic Laboratories in RochesterMethod Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Method Description
Deuterated stable isotopes (d4-cortisol, d7-androstenedione, d8 17-hydroxyprogesterone) are added to the serum sample as internal standards. Cortisol, androstenedione, 17-hydroxyprogesterone, and the internal standards are extracted from specimens online using a guard cartridge. The analytes are transferred online to an analytical column and are analyzed by liquid chromatography tandem mass spectrometry.(Unpublished Mayo method)
Reference Values
PEDIATRICS*
Premature infants
26-28 weeks, day 4: 92-282 ng/dL
31-35 weeks, day 4: 80-446 ng/dL
Full-term infants
1-7 days: 20-290 ng/dL
1 month-1 year: <69 ng/dL
Males*
Tanner stages |
Age (Years) |
Reference range (ng/dL) |
Stage I (prepubertal) |
<9.8 |
<51 |
Stage II |
9.8-14.5 |
31-65 |
Stage III |
10.7-15.4 |
50-100 |
Stage IV |
11.8-16.2 |
48-140 |
Stage V |
12.8-17.3 |
65-210 |
Females*
Tanner stages |
Age (Years) |
Reference range (ng/dL) |
Stage I (prepubertal) |
<9.2 |
<51 |
Stage II |
9.2-13.7 |
42-100 |
Stage III |
10.0-14.4 |
80-190 |
Stage IV |
10.7-15.6 |
77-225 |
Stage V |
11.8-18.6 |
80-240 |
*Soldin SJ, Brugnara C, Wong EC, eds. Androstenedione. In: Pediatric Reference Ranges. 4th ed. AACC Press; 2003: 32-34
ADULTS
Males: 40-150 ng/dL
Females: 30-200 ng/dL
For SI unit Reference Values, see www.mayocliniclabs.com/order-tests/si-unit-conversion.html
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
82157
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
ANST | Androstenedione, S | 1854-9 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
7730 | Androstenedione, S | 1854-9 |
Testing Algorithm
For more information see Steroid Pathways.
Special Instructions
Report Available
2 to 5 daysReject Due To
Gross hemolysis | Reject |
Gross lipemia | Reject |
Gross icterus | OK |