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HelpTest Code LAB523 Estradiol
Clinical System Name
Estradiol Level
Sample Requirements
Specimen: Whole blood
Container(s): Gold SST, Red, Dark Green/Sodium Heparin, Lavender/EDTA, Gold Microtainer, Lt. Green/ Lithium Heparin Microtainer
Preferred Vol: 2.0 mL
Minimum Vol: 1.0 mL
Note: This test requires a minimum of 0.5 mL of serum or plasma for testing.
Note: Capillary collection is unacceptable for estradiol testing.
Processing Instructions
Reject due to:
Spin: Y
Aliquot: Y
Temp: -20C
Storage location: CPA freezer, Fz6 rack. Serum/plasma should be removed from separator within 2 hours prior to storage.
Off-site collection: Spin blood. Transfer serum/plasma to plastic aliquot container. Transport specimen frozen.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| gel separator with serum/plasma | Refrigerated | 2 h |
| separated serum/plasma | Room temp | |
| Refrigerated | 14 d | |
| Frozen -20 C | 1 m |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Chemistry
Location: Chem West
Phone Number: (206) 987-2617
Synonyms
Estradiol, Ultra-Sensitive (US); Estradiol (E2); Ultrasensitive Estradiol
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | W, F | 2-6 d |
Test runs overnight, results available next business day
Methodology
Method: Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry
Analytical Volume: 0.5 mL serum or plasma
Limitations:
Reference Range
| Age | Estradiol--Female | Age | Estradiol--Male | |
|---|---|---|---|---|
| (Years) | (pg/mL) | (Years) | (pg/mL) | |
| 7 - 9 | < 36 | 7 - 9 | < 7 | |
| 10 - 12 | 1 - 87 | 10 - 12 | < 11 | |
| 13 - 15 | 9 - 249 | 13 - 15 | 1 - 36 | |
| 16 - 17 | 2 - 266 | 16 - 17 | 3 - 34 | |
| >18 | 10 - 42 |
| Females 18 years and older, pre-menopausal | Estradiol (pg/mL) |
|---|---|
| Early Follicular |
30.0 - 100.0 |
| Late Follicular |
100.0 - 400.0 |
| Luteal | 50.0 - 150.0 |
CPT Codes
82670