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Test Code LAB59 Chloride Level

Clinical System Name

Chloride Level

Description

Chloride is increased in dehydration, renal tubular acidosis (hyperchloremia metabolic acidosis), and in excessive infusion of isotonic saline. Chloride is decreased in overhydration, chronic respiratory acidosis, salt-losing nephritis, metabolic alkalosis, and congestive heart failure.

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Green/Mint Top Lithium Heparin, Lt. Green/Lithium Heparin Microtainer, Gold SST, Gold Microtainer, Dark Green/Sodium Heparin, Red, or Heparinized Syringe

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL

 

Note:  Method is affected by EDTA and prolonged exposure to RBCs. Specimen must be spun and aliquot within 2 hours. Not affected by Na, Li or Ammon. heparin.

Processing Instructions

Reject due to: Age of specimen, incorrect specimen collection (No EDTA).

Spin: Y

Aliquot: Y

Storage location: Core 5 Chemistry Refrigerator

 

Off-site collection: Specimen should be centrifuged within 2 hours of collection. Spin blood and transfer serum or plasma to plastic tube and refrigerate or freeze.

Stability

Specimen Type Temperature Time
Serum or Plasma RT

≤ 7 d

Serum or Plasma 2-8 C

≤ 28 d

Serum or Plasma -20 C or -70 C

≤ 365 d

Performing Laboratory

Seattle Children's Laboratory    

Department

Department: Core Chemistry

Phone Number: 206-987-2617 (Client Services)

Synonyms

LAB59

Cl - Blood (Chloride - Blood)

Availability

STAT Performed TAT
Y 24/7 1 h

Methodology

Method: Potentiometric method by Vitros 4600

Analytical Volume: 0.05 mL serum or plasma

Limitation: Potassium Bromide causes positive interference with assays that use direct or indirect ion selective electrodes.                                     

Reference Range

 Age mEq/L
<1 y   96 - 110
1 y - adult   96 - 109

CPT Codes

82435

Critical Values

≤ 70 mEq/L

≥ 130 mEq/L