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Test Code LAB60 Cholesterol

Important Note

Patient should be fasting (at least 8 hours). For patients <2 years, fasting is 4 hours.

Clinical System Name

Cholesterol

Synonyms

LAB60

CHOL

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Green/Mint Top Lithium Heparin, Lt. Green/Lithium Heparin Microtainer, Gold SST, Gold Microtainer, Dark Green/Sodium Heparin, Red

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL

 

Note:  Method is not affected by hemolysis (hemoglobin <300 mG/dL), icterus (bilirubin <30 mG/dL) or lipemia (triglycerides <200 mG/dL).  

Processing Instructions

Reject due to: Age of specimen

Spin: Y

Aliquot: Y

Storage location: Core 5 Chemistry Refrigerator

 

Off-site collection:  Patient should be fasting. Specimen should be centrifuged within 2 hours of collection. Spin blood and transfer serum or plasma to plastic tube and refrigerate or freeze.

Stability

Specimen Type Temperature Time
Serum or Plasma RT ≤ 3 h
Serum or Plasma 2-8 C

≤ 3 d
Serum or Plasma -20 C or -70 C

≤ 21 d

Availability

STAT Performed TAT
Y 24/7 1 h

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Core Chemistry

Phone Number: 206-987-2617 (Client Services)

CPT Codes

82465

Methodology

Method: Colorimetric method by Vitros 4600

Analytical Volume:  0.02 mL serum or plasma + 0.03 mL dead space

Reference Range

 Age Fasting (mG/dL)
0 - 1 M 50 - 120
> 1 M 125 - 200