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Test Code LAB61 Cortisol

Clinical System Name

Cortisol Level (LAB61)

 

Sample Requirements

Specimen: Whole blood

Container(s): Gold SST, Dark Green/Sodium Heparin, Lt. Green/Lithium Heparin Microtainer, Gold Microtainer, Red

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL

Note: Capillary collection is unacceptable for cortisol testing. 

Processing Instructions

Reject due to: 

Spin: Y

Aliquot: Y

Temp: 2-8 C

Storage location: CPA refrigerator, FrigD rack. Serum/plasma should be removed from separator for storage.

Off-site collection: Spin blood. Transfer serum/plasma to plastic aliquot container. Refrigerate.

Stability

Specimen Type Temperature Time
gel separator with serum/plasma Refrigerated 8 h
separated serum/plasma Room temp 2 h
  Refrigerated 14 d
  Frozen -20 C 30 d

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Chemistry

Location: Chem West

Phone Number:  (206) 987-2617

 

 

 

Availability

STAT Performed TAT
Y* M-Sa, day shift 1-3 d

For STAT requests, outside of testing availability, attending providers should page the Lab Medicine Attending on-call to arrange with Core Lab.

Methodology

Method: Chemiluminescent microparticle immunoassay (CMIA) on the Alinity i

Analytical Volume: 0.150 mL serum/plasma

Limitations: 

Reference Range

Cortisol mcg/dL
Baseline (8-10 AM)  3.7 -19.4
Post ACTH 30 min > 13.2
Post ACTH 60 min > 14.5

 (4-6 PM) Cortisol value is 1/2 of AM Cortisol value. 

 

CPT Codes

82533