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Test Code LAB744 Pyruvate

Important Note

Place the sample in a DRY bag, then place this bag into a bag of ice and send to the lab within 5 min. of collection. Note whether sample is venous or arterial (venous is preferable unless an arterial line is in place).

Deliver to CORE within 2 min of receipt in Lab. A protein-free filtrate must be made by CORE as soon as possible.  

Clinical System Name

Pyruvate

Sample Requirements

Specimen: Whole blood

Container(s): Dark Green/Sodium Heparin, Gray/Sodium Fluoride, Heparinized Syringe

Preferred Vol: 1 mL

Minimum Vol: 1 mL

 

Note: Place the sample in a DRY bag, then place this bag into a bag of ice and send to the lab within 5 min. of collection. Note whether sample is venous or arterial (venous is preferable unless an arterial line is in place). Note also if the patient was crying or it was a difficult draw.

 

Processing Instructions

Reject due to: Specimens received not on ice, age of specimen, insufficient volume.

Spin: N

Aliquot: N

Temp: - 20 C

Storage location: Deliver to CORE within 2 min of receipt. A protein-free filtrate must be made by CORE as soon as possible.  

 

CORE: Add 0.5 mL well-mixed whole blood to 1.0 mL cold 6% perchloric acid. Immediately mix by vortexing until the sample is chocolate brown. Centrifuge sample and aliquot the clear supernatant into a 4ml tube and labeled with the large Epic label.  Place aliquot in the "return to CPA" box.

 

CPA: Retrieve clear supernatant aliquot from CORE  and place in CPA freezer, Fz5 rack.

 

 

Off-site collection:  Draw at least 1 mL blood in a Dark Green/Sodium Heparin or Gray/Sodium Fluoride, no serum separator. Note if patient was crying or draw was difficult. Prepare protein-free filtrate: Add 0.5 mL well-mixed whole blood to 1.0 mL cold 6% perchloric acid (PCA). Mix by vortexing until the sample is a chocolate brown color. Centrifuge and transfer supernate to plastic tube.  Send frozen.

 

If PCA is unavailable, send whole blood in a gray top tube on ice within 2 hours.

Stability

Specimen Type Temperature Time
Whole blood (Green/Sodium Heparin) Room temp 5 min.
  Refrigerated 1 h
  Frozen N
Whole blood (Gray/Sodium Fluoride) Refrigerated 2 h
Protein-free filtrate, plasma Room temp 4 d
  Refrigerated 4 d
  Frozen 4 d

 

Availability

STAT Performed TAT
N M, W, F 1-4 D

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Chemistry

Location: Chem East

Phone Number: (206) 987-2617

 

Methodology

Method: Enzymatic Rate Reaction by Vitros 4600

Analytical Volume: 0.5 mL

Reference Range

 

Fasting Venous 0.03 - 0.10 mMol/L

 

 

Arterial concentrations will be slightly lower.

 

CPT Codes

84210