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Test Code LAB758 Factor 10 Activity

Important Note

This test is often confused with the Anti Factor Xa (Low Molecular Weight Heparin) Test. Patients on hirudin (Refludan), bivalirudin, and argatroban (Novastan) may show prolonged clotting times due to the direct thrombin inhibition. This may cause an under-estimation of the factor concentrations.

Clinical System Name

Factor 10 Activity

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (1) 2.7 mL

Minimum Vol: (1) 1.8 mL

 

Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended. Please review the Coagulation Lab Collection Job Aid for detailed instructions. 

Processing Instructions: Main Campus

Deliver whole blood to Coag bench. Coag Technologist will process.

 

Reject due to: clotted, hemolyzed, insufficient quantity (underfill), or improper collection (overfill).

Spin: Y

Aliquot: Y

Temp: -70 C

Storage Location: CPA 2 Freezer ( -70 C) Send Out rack.

 

Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

Processing Instructions: Offsite & Regional Clinics

Reject due to: clotted, insufficient quantity (underfill), or improper collection (overfill).

Spin: Y

Aliquot: Y

Storage location:  -70 C (preferred) or -20 C. 

 

Specimen should be centrifuged within one hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large sample label. Freeze one aliquot of plasma at -70 C (preferred) or -20 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

 

Non-Children's Hospital Offsite collection: Ship completely frozen on dry ice.

 

Children's Hospital Regional Clinic collection: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.

Stability

Specimen Type Temperature Time
Citrated platelet-poor plasma Room temp N
  Refrigerated N
  Frozen Y

 

Performing Laboratory

Harborview Medical Center

Clinical Coagulation Lab

325 9th Ave

Room GWH 47

Seattle, WA 98195

 

Phone Number: (206) 744-3128

Department

Department: Send Outs

 

Phone Number: (206) 987-2563

Send Out Instructions

Reference Test Name: Factor X Activity
Reference Test Number: F10
Instructions: Send out Monday - Friday with the UW courier.

 

Synonyms

Factor X

Factor X Activity

Availability

STAT Performed TAT
N Drawn daily, Performed M - F at HMC Coag Lab 1 - 3 d

 

Methodology

Method:  Electromagnetic mechanical clot detection assay by STA-R MAX

Analytical Volume: 500 uL plasma

Limitations: Method is affected by hemolysis, insufficient quantity (underfill), improper collection (overfill), improper processing, and improper storage. High hematocrit greater than or equal to 56% requires a citrate adjusted tube.

Reference Range

Units: %
Female Male
Age Range Age Range
0 - 29d 19 - 150 0 - 29d 19 - 150
1m - 2m 31 - 150 1m - 2m 31 - 150
3m - 5m 35 - 150 3m - 5m 35 - 150
6m - 11m 38 - 150 6m - 11m 38 - 150
1y - 50 - 150 1y - 50 - 150

 

CPT Codes

85260