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HelpTest Code LAB766 Heparin-Induced Platelet Antibody
Additional Codes
HIPA
Clinical System Name
Heparin-Induced Platelet Antibody
Synonyms
Heparin Induced Ab; HIPA; HIT Antibody; HIT Elisa; Heparin Induced Antibody; Heparin Induced Thrombocytopenia; Heparin-PF4 Antibody; PF4 Heparin Antibody
Description
Heparin-induced thrombocytopenia (HIT) is inappropriate formation of blood clots caused by an adverse drug reaction with heparin. There are several published rating systems to estimate the probability that a patient is positive for HIT. Please refer to UW Medicine Anticoagulation Services for more information.
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Blue/Citrate
Preferred Vol: (1) 2.7 mL
Minimum Vol: (1) 1.8 mL
Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended. Please review the Coagulation Lab Collection Job Aid for detailed instructions.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole blood | Room Temp |
4 h |
| Plasma | Refrigerated | N |
| Frozen |
1 m |
Availability
| STAT | Performed | TAT |
|---|---|---|
| Y, with UW Lab Med Resident (LMR) approval. |
Daily |
1 - 3 d |
Note: Samples received before noon run the same day. To get STAT approval, contact the Send Outs department at ext. 7-2563. After hours or on weekends, contact Central Processing at ext. 7-2102.
Performing Laboratory
University of Washington
Department of Laboratory Medicine
Coagulation
1959 NE Pacific St, NW220
Seattle, WA 98195
Phone Number: (206) 520-4600
Department
Department: Send Outs
Phone Number: (206) 987-2563
Methodology
Method: EIA
Analytical Volume: 500 uL plasma
Limitations: Method is affected by hemolysis, insufficient quantity (underfill), improper collection (overfill), improper processing, and improper storage. High hematocrit greater than or equal to 56% requires a citrate adjusted tube.
Reference Range
| Normal Value | Critical Value | Strong Positive |
| Negative | Positive | Elevated Thrombotic Risk |
CPT Codes
86022
Processing Instructions: Main Campus
Deliver whole blood to Coag bench. Coag Technologist will process.
Reject due to: clotted, gross hemolysis, insufficient quantity (underfill), or improper collection (overfill).
Spin: Y
Aliquot: Y
Temp: -70 C
Storage Location: CPA 2 Freezer ( -70 C) Send Out rack.
Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Send Out Instructions
| Reference Test Name: |
Heparin Induced Platelet Antibody |
| Reference Lab Test Code: |
HIPAB |
| Instructions: |
Send out Monday through Friday with UW courier. For STAT samples, ship on dry ice via Delivery Express courier to NW220. |
Processing Instructions: Offsite & Regional Clinics
Reject due to: clotted, gross hemolysis, insufficient quantity (underfill), or improper collection (overfill).
Spin: Y
Aliquot: Y
Storage location: -70 C (preferred) or -20 C.
Specimen should be centrifuged within one hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large sample label. Freeze one aliquot of plasma at -70 C (preferred) or -20 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Non-Children's Hospital Offsite collection: Ship completely frozen on dry ice.
Children's Hospital Regional Clinic collection: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.