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HelpTest Code LAB766 Heparin-Induced Platelet Antibody
Additional Codes
HIPA
Clinical System Name
Heparin-Induced Platelet Antibody
Synonyms
Heparin Induced Ab; HIPA; HIT Antibody; HIT Elisa; Heparin Induced Antibody; Heparin Induced Thrombocytopenia; Heparin-PF4 Antibody; PF4 Heparin Antibody
Description
Heparin-induced thrombocytopenia (HIT) is inappropriate formation of blood clots caused by an adverse drug reaction with heparin. There are several published rating systems to estimate the probability that a patient is positive for HIT. One such system is the 4T score: https://depts.washington.edu/anticoag/home/content/pre-test-probability-scoring-hit.
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Blue/Citrate
Preferred Vol: (1) 2.7 mL
Minimum Vol: (1) 1.8 mL
Note: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended.
If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of a blood transfer device to ensure proper fill.
Specimen should be processed within one hour of collection.
Processing Instructions
Deliver blood to Core Coag bench. Coag Technologist will process.
Reject due to: Clotted, hemolyzed, insufficient quantity, or improper collection.
Spin: Y
Aliquot: Y
Temp: -70 C
Storage Location: CPA 2 Freezer ( -70 C) Send Out rack.
Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.
Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole blood | Room Temp |
4 h |
| Plasma | Refrigerated | N |
| Frozen |
1 m |
Availability
| STAT | Performed | TAT |
|---|---|---|
| Y, with UW Lab Med Resident (LMR) approval. |
Daily |
1 - 3 d |
Note: Samples received before noon run the same day. To get STAT approval, contact the Send Outs department at ext. 7-2563. After hours or on weekends, contact Central Processing at ext. 7-2102.
Performing Laboratory
University of Washington
Department of Laboratory Medicine
Coagulation
1959 NE Pacific St, NW220
Seattle, WA 98195
Phone Number: (206) 520-4600
Department
Department: Send Outs
Phone Number: (206) 987-2563
CPT Codes
86022
Methodology
Method: EIA
Analytical Volume: 0.5 mL plasma
Limitations: Method is affected by hemolysis and improper specimen collection/processing.
Reference Range
| Normal Value | Critical Value | Strong Positive |
| Negative | Positive | Elevated Thrombotic Risk |
Send Out Instructions
| Reference Test Name: |
Heparin Induced Platelet Antibody |
| Reference Lab Test Code: |
HIPAB |
| Instructions: |
Send out Monday through Friday with UW courier. For STAT samples, ship on dry ice via Delivery Express courier to NW220. |