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Test Code LAB792 Diphtheria Toxoid IgG Antibody, Serum

Additional Codes

DIPTH

Useful For

Determining a patient's immunological response to diphtheria toxoid vaccination

 

Aiding in the evaluation of immunodeficiency

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

Diphtheria Toxoid IgG Ab, S
Seattle Children's Hospital Note:

Clinical System Name: Diphtheria Antibody Level

Specimen Type

Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Seattle Children's Hospital Note:

Collect 1.0 mL whole blood in a red or gold top.

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 30 days
  Frozen  30 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject
Heat inactivated specimen Reject

Reference Values

Vaccinated: Positive (≥0.01 IU/mL)

Unvaccinated: Negative (<0.01 IU/mL)

Reference values apply to all ages.

Method Description

The Anti-Diphtheria Toxoid IgG enzyme-linked immunosorbent assay provides a quantitative in vitro assay for detecting human IgG-class antibodies to diphtheria toxoid. The test kit contains reagent wells coated with diphtheria toxoid. In the first reaction step, diluted patient samples are incubated in the wells. In the case of positive samples, specific IgG antibodies will bind to the antigens. To detect the bound antibodies, a second incubation is carried out using an enzyme-labeled antihuman IgG (enzyme conjugate), catalyzing a color reaction.(Unpublished Mayo method)

Day(s) Performed

Monday through Friday

Seattle Children's Hospital Note:

TAT: 3 - 6 days

Report Available

Same day/1 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

86317

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DIPGS Diphtheria Toxoid IgG Ab, S 48654-8

 

Result ID Test Result Name Result LOINC Value
DIPG Diphtheria IgG Ab 45166-6
DEXDP Diphtheria IgG Value 48654-8

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.