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HelpTest Code LAB827 Tryptase, Serum
Additional Codes
Tryptase
Reporting Name
Tryptase, SSCH Clinical System Name: Tryptase
Useful For
Evaluation of individuals with suspected mast cell activation, which may occur as a result of anaphylaxis or allergen challenge
Evaluation of patients with suspected cutaneous or systemic mastocytosis
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial
Additional Information: Tryptase degenerates very quickly when left in the presence of red blood cells.
Collect 1.0 mL whole blood in a red top.
Specimen Type
SerumSpecimen Minimum Volume
0.2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen (preferred) | 14 days | |
| Refrigerated | 7 days |
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Method Name
Fluorescence Enzyme Immunoassay (FEIA)
Method Description
Anti-tryptase, covalently coupled to ImmunoCAP, reacts with tryptase in the patient serum specimen. After washing, enzyme-labeled antibodies against tryptase are added to form a complex. After incubation, unbound enzyme-labeled antibodies are washed away, and the bound complex is incubated with a developing agent. After stopping the reaction, the fluorescence in the eluate is measured. The fluorescence is directly proportional to the concentration of tryptase in the serum specimen.(Package insert: ImmunoCAP Tryptase. Phadia AB; 10/2019)
Reference Values
<11.5 ng/mL
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
83520
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TRYPT | Tryptase, S | 21582-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| TRYPT | Tryptase, S | 21582-2 |
Report Available
2 to 5 daysReject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Forms
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.
Testing Algorithm
For more information, see Mast Cell Disorder: Diagnostic Algorithm, Bone Marrow
Special Instructions
Aliases: Mast Cell Tryptase