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Test Code LAB855 Tetanus Toxoid IgG Antibody, Serum

Useful For

Assessing antibody response to the tetanus toxoid vaccine, which should be performed at least 3 weeks after immunization

 

Aiding in the evaluation of immunodeficiency

 

This test should not be used to diagnose tetanus infection.

Method Name

Enzyme Immunoassay (EIA)

Reporting Name

Tetanus Toxoid IgG Ab, S

Specimen Type

Serum
Seattle Children's Hospital Note:

Clinical System Name: Tetanus Antibody Level


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Seattle Children's Hospital Note:

Collect 1.0 mL whole blood in a red or gold top.

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 30 days
  Frozen  30 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Reference Values

Vaccinated: Positive (≥0.01 IU/mL)

Unvaccinated: Negative (<0.01 IU/mL)

Method Description

The Anti-Tetanus Toxoid enzyme-linked immunosorbent assay provides a quantitative in vitro assay for detecting human IgG-class antibodies to Tetanus toxoid. The test kit contains reagent wells coated with tetanus toxoid. In the first reaction step, diluted patient samples are incubated in the wells. In the case of positive samples, specific IgG antibodies will bind to the antigens. To detect the bound antibodies, a second incubation is carried out using an enzyme-labeled anti-human IgG (enzyme conjugate), catalyzing a color reaction.(Unpublished Mayo method)

Day(s) Performed

Monday through Friday

Seattle Children's Hospital Note:

TAT: 3 - 6 days

Report Available

Same day/1 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

86317

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TTIGS Tetanus Toxoid IgG Ab, S 53935-3

 

Result ID Test Result Name Result LOINC Value
TETG Tetanus IgG Ab 26643-7
DEXTG Tetanus IgG Value 53935-3

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.