Home
HelpTest Code LAB869 HLA B27 Antigen
Clinical System Name
HLA-B27 Antigen
Synonyms
Tissue Typing – HLA B 27
HLA B27
HLA-B27
HLA B27 Antigen
Sample Requirements
Specimen: Whole Blood
Container(s): Lavender/EDTA
Preferred Vol: 1 mL
Minimum Vol: 0.5 mL
Note: Confirmatory testing of equivocal results is available at Bloodworks Northwest. If confirmatory testing is requested, please place a CIS add-on for a MISC test. In the order comments, please enter "HLA B27 Confirmatory to BWNW". CPA: please add testing to original HLA B27 container (Lavender/EDTA) found in Cell Markers and deliver to Send Outs room temp rack. Sample stable for one week at room temperature.
Processing Instructions
Reject due to: n/a - send to lab
Spin: N
Aliquot: N
Temp: RT
Storage location: Days: Transport specimen and labels to Cell Markers lab (station #181). Eves/Nights: Store specimen and labels in the Cell Markers RT box in CPA.
Off-site collection: Keep whole blood at room temperature and transport to Seattle Children's.
Stability
| Temperature | Time |
|---|---|
| Room temp | 72 hours |
| Refrigerated |
N |
| Frozen | N |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | collected daily; performed M-Sat | 2-3 days |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Cell Markers
Phone Number: 206-987-2560
CPT Codes
| CPT CODE |
|---|
| 86812 |
Methodology
Method: Flow Cytometry
Analytical Volume: 200 uL whole blood
Limitations: Method is affected by age of sample, heat and cold.
Reference Range
| Negative |
Found in Caucasians 6 - 8%, African-Americans 3 - 4%, and Asians 1%.
Description
This qualitative assay is used to identify the B27 Human Leukocyte Antigen. The presence of the HLA-B27 antigen in a patient with symptoms has been found to be highly associated with Ankylosing Spondylitis and related disorders.