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HelpTest Code LAB875 Sirolimus Level
Additional Codes
SIROL
Clinical System Name
Sirolimus Level
Synonyms
Rapamune
Rapamycin
Sample Requirements
Specimen: Whole blood
Container(s): Lavender/EDTA or Lavender/EDTA microtainer
Preferred Vol: 1 mL
Minimum Vol: 0.5 mL
Note: Trough levels are collected within one hour of the next dose. Please arrive to the lab 30 – 60 min before the next dose time to allow for probable wait times.
Specimen must be received in the lab by 10:00 for same day results.
If patient is receiving IV sirolimus, the lab sample must be drawn off a separate line or from a peripheral source. Assay can also be performed on dried bloodspots, but must order Sirolimus Level Blood Spot.
Processing Instructions
Reject due to: Frozen specimen.
Spin: N
Aliquot: N
Temp: Store at room temperature.
Storage location: Store specimen and labels in RT1 box.
Off-site collection:Trough levels are collected within one hour of the next dose. Note draw time on the requisition. If patient is receiving IV sirolimus, the lab sample must be drawn off a separate line or from a peripheral source. Do not centrifuge. Send refrigerated if sample will not be received in lab within 24 hours. Assay can also be performed on dried bloodspots, but must order Sirolimus Level Blood Spot.
Stability
| Temperature | Time |
|---|---|
| Room temp | 2 d |
| Refrigerated | 7 d |
| Frozen | N |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | Daily |
Daily results report out by 14:00 if received in lab by 10:00
Specimens received between 10:00 and 13:00 |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Chemistry
Location: Chem West
Phone Number: (206)987-2617
CPT Codes
80195
Methodology
Method: LC/MS/MS
Analytical Volume: 50 mcL whole blood
Limitations:
Reference Range
Trough Level 5 - 20 ng/mL