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HelpTest Code LAB90 Hemoglobin A1C
Clinical System Name
Glycosylated Hemoglobin A1C
Synonyms
HbA1c
HB A1C
Sample Requirements
Specimen: Whole blood
Container(s): Lavender/EDTA
Preferred Vol: 1.5 mL
Minimum Vol: 1.0 mL
Note: Do not overfill microtainer. Mix by inverting 10 times. Due to analytical testing volume, two microtainers are preferred.
Processing Instructions
Reject due to:
Spin: N
Aliquot: N
Temp: 2 - 8 C
Storage location: CPA refrigerator, FrigA rack.
Off-site collection: Send whole blood refrigerated. Do not spin or aliquot.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| whole blood | Room temp | 3 d |
| Refrigerated | 7 d | |
| Frozen | 2 w |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | M-F, day shift | 1 - 3 d |
Contact Chemistry Lab for requests outside of stated availability (206) 987-3694 or (206) 987-2565 for weekend/holiday days.
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Chemistry
Location: Chem East
Phone Number: (206) 987-2617
CPT Codes
83036
Methodology
Method: Immunoassay on Vitros 4600
Analytical Volume: 0.350 mL
Limitations:
Reference Range
ADA guidelines suggest that an HbA1c of <5.7% is normal, 5.7-6.4% is increased risk of diabetes (prediabetes), and >6.4% indicates diabetes.
The DCA Vantage (Point of Care instrument) has a positive bias compared to Ortho Vitros 4600 and BioRad HPLC (gold standard) of approximately 4.8%.