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HelpTest Code LAB96 Lactate Dehydrogenase
Clinical System Name
Lactate Dehydrogenase
Synonyms
LDH - Blood
LD
Sample Requirements
Specimen: Whole blood
Container(s): Lt. Green/Mint Top Lithium Heparin, Lt. Green/Lithium Heparin Microtainer, Gold SST, Gold Microtainer, Dark Green/Sodium Heparin, Red
Preferred Vol: 1.0 mL
Minimum Vol: 0.5 mL
Note: Method is affected by oxalate, hemolysis, and by exposure to RBC's. A slow freeze and thaw can result in a loss of up to 70% of LDH activity. Not affected by lipemia.
Processing Instructions
Reject due to: Age of specimen, incorrect collection container, previously frozen
Spin: Y
Aliquot: Y
Storage location: Core 5 Refrigerator
Off-site collection: Avoid hemolysis. Specimen should be centrifuged within 1 hour of collection. Spin blood and transfer serum or plasma to plastic tube and keep at room temp. Transport specimen at room temperature. Samples are stable for 2 days at room temperature.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum or Plasma | RT |
≤ 2 d |
| Serum or Plasma | 2-8 C |
≤ 8 h |
| -20 C or -70 C |
N |
Availability
| STAT | Performed | TAT |
|---|---|---|
| Y | 24/7 | 1 h |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Core Chemistry
Phone Number: 206-987-2617 (Client Services)
CPT Codes
83615
Methodology
Method: Multiple Point Rate by Vitros 4600
Analytical Volume: 0.02 mL serum or plasma + 0.03 mL dead space
Reference Range
New ranges, effective Saturday Sept 10, 2022:
|
Age |
IU/L |
| 0- 1 W | Up to 957 |
| 1 W- 5 Y | 151 - 408 |
| 5 - 12 Y | 125 - 345 |
| 12 - 14 Y | 125 - 319 |
| 14 - 16 Y | 125 - 253 |
| >16 Y | 98 - 241 |
Description
Causes of high lactate dehydrogenase include neoplastic states, hypoxic cardio respiratory diseases, myocardial infarctions, hemolytic anemias, megaloblastic anemias, hepatic cirrhosis, renal infarction, trauma, muscle damage muscular dystrophy, shock, and hypotension. In myocardial infarction cases, LDH begins to rise within about 12 hours after infarction and usually returns to baseline after 2 to 5 days.