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Test Code LAB977 Dihydrotestosterone, Serum

Additional Codes

DIHYDROTES

Reporting Name

Dihydrotestosterone, S
Seattle Children's Hospital Note:

SCH Clinical System Name: Dihydrotestosterone

Useful For

Monitoring patients receiving 5-alpha reductase inhibitor therapy or chemotherapy

 

Evaluating patients with possible 5-alpha reductase deficiency


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Seattle Children's Hospital Note:

Collect 2.0 mL whole blood in a Red top.

Specimen Type

Serum

Specimen Minimum Volume

0.6 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 28 days
  Frozen  90 days
  Ambient  28 days

Day(s) Performed

Monday, Wednesday, Friday

Seattle Children's Hospital Note:

TAT: 4 - 10 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Portions of this test are covered by patents held by Quest Diagnostics

Method Description

The assay uses high performance liquid chromatography with triple quadrupole tandem mass spectrometry. Deuterated stable isotope of dihydrotestosterone (13C3-DHT) is added to a 0.5 mL serum sample as internal standard. The DHT and internal standard are extracted from the sample by liquid/liquid extraction. This is followed by high performance liquid chromatography on a Cohesive LX4 System and analysis on a tandem mass spectrometer equipped with an electrospray ion source.(Unpublished Mayo Method)

Reference Values

Males

Cord blood: ≤100 pg/mL

≤6 months: ≤1,200 pg/mL

Tanner Stages

Mean

Age

Reference range (pg/mL)

Stage I (>6 months and prepubertal)

7.1 years

≤50

Stage II

12.1 years

≤200

Stage III

13.6 years

80-330

Stage IV

15.1 years

220-520

Stage V

18 years

240-650

>19 years: 112-955 pg/mL

 

Females

Cord blood: ≤50 pg/mL

≤6 months: ≤1,200 pg/mL

Tanner Stages

Mean

Age

Reference range (pg/mL)

Stage I (>6 months and prepubertal)

7.1 years

≤50

Stage II

10.5 years

≤300

Stage III

11.6 years

≤300

Stage IV

12.3 years

≤300

Stage V

14.5 years

≤300

20-55 years: ≤300 pg/mL

>55 years: ≤128 pg/mL

 

1. Pang S, Levine LS, Chow D, Sagiani F, Saenger P, New MI. Dihydrotestosterone and its relationship to testosterone in infancy and childhood. J Clin Endocrinol Metab. 1979;48(5):821-826

2. Stanczyk FZ. Diagnosis of hyperandrogenism: biochemical criteria. Best Pract Res Clin Endocrinol Metab. 2006;20(2):177-191

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

82642

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DHTS Dihydrotestosterone, S 1848-1

 

Result ID Test Result Name Result LOINC Value
81479 Dihydrotestosterone, S 1848-1

Testing Algorithm

For more information see Steroid Pathways.

Special Instructions

Report Available

2 to 8 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK
Seattle Children's Hospital Additional Information:

Aliases: 5-a-dihydrotestosterone; 5-Alpha-Dihydrotestosterone; Allodihydrotestosterone; Androstanolone; DHT; Serum Dihydrotestosterone; Stanolone