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HelpTest Code LAB979 Pancreatic Elastase, Feces
Additional Codes
ELASTASE
Shipping Instructions
Preferred shipping temperature is frozen. Refrigerated or ambient specimens received more than 72 hours post collection will be rejected.
Specimen Required
Supplies: Stool container, Small (Random), 4 oz (T288)
Container/Tube: Stool container
Specimen Volume: 5 g
Collection Instructions:
1. Collect a fresh random fecal specimen, no preservatives.
2. If specimen is sent refrigerate, send immediately after collection.
3. If specimen cannot be sent immediately, freeze specimen and send frozen.
Additional Information:
1. Separate specimens must be submitted when multiple tests are ordered, with the exception of CALPR / Calprotectin, Feces. If only a single specimen is collected, it must be split prior to transport.
2. Testing cannot be added on to a previously collected specimen.
Useful For
Diagnosis of exocrine pancreatic insufficiency in case of unexplained diarrhea, constipation, steatorrhea, flatulence, weight loss, upper abdominal pain, and food intolerances
Monitoring of exocrine pancreatic function in cystic fibrosis, diabetes mellitus, or chronic pancreatitis
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Reporting Name
Pancreatic Elastase, FSCH Clinical System Name: Pancreatic Elastase, Fecal
Specimen Type
FecalSpecimen Minimum Volume
1 g
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Fecal | Frozen (preferred) | 28 days | |
| Ambient | 72 hours | ||
| Refrigerated | 72 hours |
Reject Due To
| Specimens collected from diapers | Reject |
Reference Values
<100 mcg/g (Severe Pancreatic Insufficiency)
100-200 mcg/g (Moderate Pancreatic Insufficiency)
>200 mcg/g (Normal)
Reference values apply to all ages.
Method Description
The Immundiagnostik Pancreatic Elastase assay is an enzyme-linked immunosorbent assay (ELISA). Calibrators, controls, and diluted patient samples are added to a 96-well plate pre-coated with monoclonal antibodies to pancreatic elastase. If present, pancreatic elastase will bind to the antibodies on the surface of the microtiter wells. After a wash step, a peroxidase-labeled conjugate (mouse anti-pancreatic elastase) is added. After another washing step, substrate tetramethylbenzidine is added, which reacts with the peroxidase. An acidic stop solution is added causing the color to change from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of pancreatic elastase. A dose response curve of absorbance unit (optical density at 450 nm) vs. concentration is generated using the values obtained from the standards. Pancreatic elastase present in the patient samples is determined directly from this curve.(Package insert: IDK Pancreatic Elastase ELISA kit. Immundiagnostik AG; 2022)
Day(s) Performed
Monday through Friday
Report Available
3 to 5 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
82653
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ELASF | Pancreatic Elastase, F | 25907-7 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| ELASF | Pancreatic Elastase, F | 25907-7 |
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-General Request (T239)
Aliases: Pancreatic Elastase-1; Elastase-1; EL-1; FE-1; PE-1; Fecal Elastase; Elastase; PE Stool