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HelpTest Code Lymphocyte Subset Analys. Leukocyte Adhesion Defect Panel
Additional Codes
MULTIPLE PANEL SELECTIONS:
Ordering Providers: select desired tests on requisition or in CIS - see table below.
| Clinical System Name | Panel Information | Lab Orderable |
|---|---|---|
| Leukocyte Adhesion Defect Full Panel | LAD - CD11a, CD11b, CD11c, CD18 | CD18/CD11abc |
| Leukocyte Adhesion Study CD18 Only | LAD - CD18 Only | CD18 Only |
Panels can be ordered alone, or in combination with other T and B Panels - duplicate tests will not be charged.
Clinical System Name
Multiple selections - see chart above
Synonyms
CD18 Only
CD18/CD11abc
LAD CD18 Only
LAD Full Panel CD18, CD11abc
Leukocyte Adhesion Defect Full Panel
Leukocyte Adhesion Study CD18 Only
Lymphocyte Subset
Lymphocyte Subset Analysis
Sample Requirements
Specimen: Whole Blood
Container(s): Lavender/EDTA; Dk Green/Na Heparin also acceptable from referring labs if CBC & differential results submitted with it
Preferred Vol: 2-3 mL
Minimum Vol: 2 mL; no microtainers
| Collected in-house at Seattle Children's | Collected off-site (including SCH Bellevue, SCH North and SCH South) | |
|---|---|---|
| M-Th | collect any time | collect any time |
| Friday | collect any time | ok if received at SCH main lab same day, if not do not collect |
| Saturday | ok if collected before 12 noon | do not collect |
| day before a holiday | ok if collected before 12 noon | must be received at SCH main lab before 12 noon |
| Sunday | ok if collected after 12 noon (to be tested next morning) |
ok if collected after 12 noon (to be tested next morning) |
| Sunday before a holiday Monday | do not collect | do not collect |
| Holiday | ok if collected after 12 noon (to be tested next morning) | ok if collected after 12 noon (to be tested next morning) |
Children's Outpatient Blood Draw areas and Community Services - order "Lymphocyte Subset" and select Paper Requisition under order comments (specific tests will be ordered in Cell Markers Lab) and submit copy of requisition with specimen.
Processing Instructions
Reject due to: clotted specimen, capillary collection, refrigerated
Spin: N
Aliquot: N
Temp: RT - DO NOT REFRIGERATE
Note: Have Core Lab run specimen through hematology analyzer and send a copy of the results and community service requisition (if applicable) with specimen to Cell Markers.
Storage location: Days: Transport specimen, copy of community service requisition (if applicable), and labels to the Cell Markers Lab (station #181). Eves/Nights: Store specimen, copy of community service requisition (as applicable), and labels in the Cell Markers RT box in CPA.
Off-site collection: Keep whole blood at room temperature. Referring labs may send CBC w/differential results from the same collection along with the sample. Transport specimens to Seattle Children's Hospital address on requisition: Laboratory OC.8.720, 4800 Sand Point Way NE, Seattle, WA 98105.
Stability
| Temperature | Time |
|---|---|
| Room temp | EDTA - 30 hours; Heparin - 48 hours |
| Refrigerated | N |
| Frozen | N |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | M-F, Sa (0730-1200) | 2-3 days |
Note: limited specimen stability - see Sample Requirements
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Cell Markers
Phone Number: 206-987-2560
CPT Codes
| Name | Panel Information | CPT CODE(S) |
|---|---|---|
| Leukocyte Adhesion Defect Full Panel | LAD - CD11a, CD11b, CD11c, CD18 | |
| CM AB IF CD18, CD11 A, B, & C | 86356 | |
| Leukocyte Adhesion Study CD18 Only | LAD - CD18 Only | 86356 |
Methodology
Method: Flow Cytometry
Analytical Volume: Dependent on patient's white count and testing requested. If <1mL is collected please ask to recollect in order to obtain minimum volume of 1mL. If unable to recollect and sample volume is <0.350mL (350uL) please cancel and do not send to Cell Markers for testing. If unable to recollect and sample is between 0.350mL and 1mL (350ul-1mL) please send to Cell Markers, but let patient and/or patient family know that there is a strong possibility that we will not be able to perform the testing due to low volume. We will do our best and understand that the volume needed is dependent on the white count and the specific antibodies that are ordered. (The higher the white count the less volume needed. The more antibodies ordered the more volume needed.)
Reference Range
Reference values accompany patient report.
Description
The leukocyte adhesion panel is used to evaluate for the presence or absence of key molecules on white blood cells that are necessary for their migration out of peripheral blood circulation and into the tissues.