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HelpTest Code Lymphocyte Subset analy. T-Cell Receptor (TCR) Study
Additional Codes
Study includes: CD3, TCR alpha/beta, TCR gamma/delta. Panels can be ordered alone, or in combination with other T and B panels. Duplicate tests will not be charged.
Clinical System Name
Lymphocyte Subsets - CD3, TCR a/b, TCR g/d
Synonyms
T cell receptor alpha-beta
T cell receptor gamma-delta
TCR Alpha Beta
TCR Gamma Delta
T cell receptor
T-cell receptor
TCR
lymphocyte subset
Sample Requirements
Specimen: Whole Blood
Container(s): Lavender/EDTA; Dk Green/Na Heparin also acceptable from referring labs if CBC & differential results submitted
Preferred Vol: 2-3 mL
Minimum Vol: 1 mL
Note: Lymphocyte subsets (T&Bs) SHOULD SHARE one lavender tube with CBC Diff (if ordered) - a minimum of 2 mL is required in this case.
| Collected in-house at Seattle Children's | Collected off-site (including SCH Bellevue, SCH North, and SCH South) | |
|---|---|---|
| M-Th | collect any time | collect any time |
| Friday | collect any time | ok if will be received at SCH main lab same day, if not do not collect |
| Saturday | ok if collected before 12 noon | do not collect |
| day before a holiday | ok if collected before 12 noon | must be received at SCH main lab before 12 noon |
| Sunday | ok if collected after 12 noon (to be tested next morning) |
ok if collected after 12 noon (to be tested next morning) |
| Sunday before a holiday Monday | do not collect | do not collect |
| Holiday | ok if collected after 12 noon (to be tested next morning) | ok if collected after 12 noon (to be tested next morning) |
Children's Outpatient Blood Draw areas and Community Services - order "Lymphocyte Subset" and select Paper Requisition under order comments (specific tests will be ordered in Cell Markers Lab) and submit copy of requisition with specimen.
Processing Instructions
Reject due to: clotted specimen, capillary collection, refrigerated
Spin: N
Aliquot: N
Temp: RT - DO NOT REFRIGERATE
Note: Have Core Lab perform a CBC with differential and send a copy of the results and community services requisition (if applicable) with specimen to Cell Markers.
Storage location: Days: Transport specimen, copy of community services requisition (if applicable), and labels to Cell Markers (tube station #181). Eves/Nights: Store specimen, copy of community services requisition (if applicable), and labels in the Cell Markers RT box in CPA.
Off-site collection: Keep whole blood at room temperature. Referring labs may send CBC w/differential results from the same collection along with the sample.
Stability
| Temperature | Time |
|---|---|
| Room temp | EDTA - 30 hours; Heparin - 48 hours |
| Refrigerated | N |
| Frozen | N |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | M-F, Sa (0730-1200) | 2-3 days |
Note: limited specimen stability - see Sample Requirements
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Cell Markers
Phone Number: 206-987-2560
CPT Codes
Contact Laboratory Client Services at (206)-987-2617
Methodology
Method: Flow Cytometry
Analytical Volume: Dependent on patient's white count and testing requested. If <1mL is collected please ask to recollect in order to obtain minimum volume of 1mL. If unable to recollect and sample volume is <0.350mL (350uL) please cancel and do not send to Cell Markers for testing. If unable to recollect and sample is between 0.350mL and 1mL (350ul-1mL) please send to Cell Markers, but let patient and/or patient family know that there is a strong possibility that we will not be able to perform the testing due to low volume. We will do our best and understand that the volume needed is dependent on the white count and the specific antibodies that are ordered. (The higher the white count the less volume needed. The more antibodies ordered the more volume needed.)
Reference Range
Reference values accompany patient report.
Description
The T Cell Receptor (TCR) assay is typically used in the evaluation of immunodeficiency and identifies the relative proportion of alpha-beta versus gamma-delta T-cells in the peripheral blood.
Note: This is not the same test as TCR Gene Rearrangement Study for Assessment of Clonality - for information regarding gene rearrangement assay, please contact Sendouts Department at 206-987-2563 and/or Pathology at 206-987-2103.