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Test Code OLIGO BAND Oligoclonal Banding, Serum and Spinal Fluid

Reporting Name

Oligoclonal Banding

Useful For

Diagnosis of multiple sclerosis; especially useful in patients with equivocal clinical presentation and radiological findings


Specimen Required


Both serum and spinal fluid are required. Spinal fluid must be obtained within 7 days of serum collection.

 

Specimen Type: Serum

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Centrifuge and aliquot serum into a plastic vial within 2 hours of collection.

2. Label specimen as serum.

 

Specimen Type: Spinal fluid

Container/Tube: Sterile vial

Specimen Volume: 0.5 mL

Collection Instructions: Label specimen as spinal fluid.


Seattle Children's Hospital Note:

Collect 1.0 mL whole blood in a Red top.

Specimen Type

CSF
Serum

Specimen Minimum Volume

Serum, Spinal Fluid: 0.4 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
CSF Refrigerated (preferred) 14 days
  Ambient  14 days
  Frozen  14 days
Serum Refrigerated (preferred) 14 days
  Ambient  14 days
  Frozen  14 days

Day(s) Performed

Monday through Friday

Seattle Children's Hospital Note:

TAT: 4 - 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Method Name

Isoelectric Focusing (IEF) with IgG Immunoblot Detection

Method Description

The oligoclonal band (OCB) assay requires paired cerebrospinal fluid (CSF) and serum samples. Unconcentrated CSF and diluted serum are electrophoresed by isoelectric focusing. The separated immunoglobulins (Ig) are visualized by an IgG immunoblot, and OCBs that are present in the CSF and not in the serum are reported. The assay uses reagents from Helena Laboratories.(Keir G, Luxton RW, Thompson EJ: Isoelectric focusing of cerebrospinal fluid immunoglobulins G: an annotated update. Ann Clin Biochem 1990;27:436-443; Hortin GL: Amino Acids, Peptides, and Proteins. In: Burtis CA, Bruns DE, eds. Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics. 7th ed. Elsevier; 2015:chap 18)

Reference Values

CSF Oligoclonal Bands Interpretation: <2 bands

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

83916 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
OLIG Oligoclonal Banding 100757-4

 

Result ID Test Result Name Result LOINC Value
8017 CSF Bands 49852-7
2783 Serum Bands 100755-8
23611 CSF Olig Bands Interpretation 100756-6

Profile Information

Test ID Reporting Name Available Separately Always Performed
OLIGS Serum Bands No Yes
OLIGC CSF Bands No Yes

Report Available

2 to 4 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK