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HelpTest Code PLP Pyridoxal 5-Phosphate, Plasma
Useful For
Determining the concentration of pyridoxal 5-phosphate in the assessment of vitamin B6 status
Method Name
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Pyridoxal 5-Phosphate (PLP), PSpecimen Type
Plasma HeparinShipping Instructions
Ship specimen in amber vial to protect from light.
Specimen Required
Patient Preparation:
1. Patient should fast overnight (12-14 hours); infants-should have specimen collected before next feeding. Water can be taken as needed.
2. For 24 hours before specimen collection, patient must not take multivitamins or vitamin supplements.
Supplies: Amber Frosted Tube, 5 mL (T915)
Collection Container/Tube: Green top (sodium or lithium heparin) or plasma gel separator (PST)
Submission Container/Tube: Amber vial
Specimen Volume: 1 mL
Collection Instructions:
1. Centrifuge at 4° C within 2 hours of collection.
2. Aliquot all plasma into amber vial and freeze immediately.
Specimen Minimum Volume
0.75 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma Heparin | Frozen | 29 days | LIGHT PROTECTED |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
5-50 mcg/L
Day(s) Performed
Monday through Thursday, Saturday, Sunday
TAT: 3 - 6 days
Report Available
1 to 7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
84207
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PLP | Pyridoxal 5-Phosphate (PLP), P | 30552-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 4047 | Pyridoxal 5-Phosphate (PLP), P | 30552-4 |
Forms
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.
Method Description
The stable isotope pyridoxal 5-phosphate-d2 and/or pyridoxic acid-d2 is added to plasma as an internal standard. Meta-phosphoric acid solution is then added to precipitate the proteins. Following sedimentation of the proteins, an aliquot of the clarified supernatant fluid is subjected to separation of pyridoxal 5-phosphate, pyridoxic acid, and internal standards from other plasma components by reverse-phase high-performance liquid chromatography with quantitation by tandem mass spectrometry.(Unpublished Mayo method)
Aliases: B6 (Vitamin); PALP (Pyridoxal 5-Phosphate); Pyridoxal 5-Phosphate (PALP); Pyridoxal Phosphate; Vitamin B6