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HelpTest Code PROT C AG Protein C Antigen
Clinical System Name
Protein C Antigen
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Blue/Citrate
Preferred Vol: (1) 2.7 mL
Minimum Vol: (1) 1.8 mL
Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended. Please review the Coagulation Lab Collection Job Aid for detailed instructions.
Processing Instructions: Main Campus
Deliver whole blood to Coag bench. Coag Technologist will process.
Reject due to: clotted, hemolyzed, insufficient quantity (underfill), or improper collection (overfill).
Spin: Y
Aliquot: Y
Temp: -70 C
Storage Location: CPA 2 Freezer ( -70 C) Send Out rack.
Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Processing Instructions: Offsite & Regional Clinic
Reject due to: clotted, insufficient quantity (underfill), or improper collection (overfill).
Spin: Y
Aliquot: Y
Storage location: -70 C (preferred) or -20 C.
Specimen should be centrifuged within one hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large sample label. Freeze one aliquot of plasma at -70 C (preferred) or -20 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Non-Children's Hospital Offsite collection: Ship completely frozen on dry ice.
Children's Hospital Regional Clinic collection: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Citrated platelet-poor plasma | Room temp | N |
| Refrigerated | N | |
| Frozen | Y |
Performing Laboratory
Harborview Medical Center
Room GWH47; 523 9th Ave
Seattle, WA 98104
Phone Number: (206) 744-3451
Department
Department: Send Outs
Phone Number: (206) 987-2563
Synonyms
Protein C Ag
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | Drawn daily; run weekly | up to 7 d |
Methodology
Method: Clot based
Analytical Volume: 600 uL plasma
Limitations: Method is affected by insufficient quantity (underfill), improper collection (overfill), improper processing, and improper storage. High hematocrit greater than or equal to 56% requires a citrate adjusted tube.
Reference Range
| Age | % |
| 0 - 1 months | 20 - 150 |
| 1 month - 3 months | 21 - 150 |
| 3 months - 6 months | 28 - 150 |
| 6 months - 1 year | 37 - 150 |
| 1 - 6 years | 40 - 150 |
| 6 - 10 years | 45 - 150 |
| 10 years - Adults | 65 - 150 |
CPT Codes
85302
Send Out Instructions
| Reference Test Name: | Protein C Antigen |
| Reference Test Number: | PCAG |
| Instructions: | Send out Monday thru Friday with the UW/HMC courier. Keep a copy of the Batch Sheet for our records. |