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Test Code PROT C AG Protein C Antigen

Clinical System Name

Protein C Antigen

Synonyms

Protein C Ag

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (1) 2.7 mL

Minimum Vol: (1) 1.8 mL

 

Note: Preferred method of collection is venipuncture with vacuum fill.  Test results are affected by incorrect blood volume.  Use of a Vascular Access Device for the  collection of coag testing is not recommended.

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill. 

Specimen should be processed within one hour of collection. 

Processing Instructions

Deliver blood to Core Coag bench. Coag Technologist will process.

 

Reject due to: Clotted, hemolyzed, insufficient quantity, or improper collection.

Spin: Y

Aliquot: Y

Temp: -70 C

Storage Location: CPA 2 Freezer ( -70 C) Send Out rack.

 

Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

 

Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device. 

Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

Stability

Specimen Type Temperature Time
Citrated platelet-poor plasma Room temp  N
  Refrigerated  N
  Frozen  Y

 

Availability

STAT Performed TAT
N Drawn daily; run weekly up to 7 d

 

Performing Laboratory

Harborview Medical Center

Department

Department:  

Harborview Medical Center

Room GWH47; 523 9th Ave
Seattle, WA 98104
 

Phone Number: (206) 744-3451

 

CPT Codes

85302

 

Methodology

Method: Clot based

Analytical Volume: 0.5 mL plasma - no result guaranteed

Limitations:

Reference Range

 

 

Age %
0 - 1 months 20 - 150
1 month - 3 months 21 - 150
3 months - 6 months 28 - 150
6 months - 1 year 37 - 150
1 - 6 years 40 - 150
6 - 10 years 45 - 150
10 years - Adults 65 - 150

 

Send Out Instructions

 

Reference Test Name: Protein C Antigen
Reference Test Number: PCAG
Instructions: Send out Monday thru Friday with the UW/HMC courier. Keep a copy of the Batch Sheet for our records.