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Test Code Plt EM Platelet Transmission Electron Microscopic Study, Whole Blood

Useful For

Diagnosing platelet disorders

Method Name

Transmission Electron Microscopy

Reporting Name

Platelet TEM, B
Seattle Children's Hospital Note:

Clinical System Name: Platelet Electron Microscopy

Specimen Type

Whole Blood ACD


Shipping Instructions


Send specimen Monday through Wednesday.

 

Specimens must be received in testing laboratory within 72 hours of collection. Ship specimen overnight in an Ambient Shipping Box-Critical Specimens Only (T668) following the instructions in the mailer.



Necessary Information


Platelet Esoteric Testing Patient Information is required. Testing may proceed without the patient information, however, the information aids in providing a more thorough interpretation. Ordering providers are strongly encouraged to fill out the form and send with the specimen.



Specimen Required


Patient Preparation: Fasting is preferred but not required. 

Supplies: Ambient Shipping Box-Critical Specimens Only (T668)

Collection Container/Tube:

Preferred: Yellow top (ACD, solution B)

Acceptable: Yellow top (ACD, solution A)

Specimen Volume: 6 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.


Specimen Minimum Volume

3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood ACD Ambient 72 hours

Reject Due To

Gross hemolysis Reject
Gross lipemia OK

Reference Values

Mean dense granules/platelet: ≥1.2

Method Description

This test identifies dense granule deficiency by semiquantitative counting of dense granules on whole mount grids by technologists and verified by a hematopathologist/hematologist.

 

Whole mount-transmission electron microscopy is a quick and simple way to examine platelet electron opaque or dense granule (DG) by laying platelet-rich plasma on an electron microscopy grid. The high content of calcium in DG blocks electron beam of transmission electron microscopy and creates a sharp dark shadow.(White JG. The dense bodies of human platelets: inherent electron opacity of the serotonin storage particles. Blood. 1969;33[4]:598-606; Winey M, Meehl JB, O'Toole ET, Giddings TH Jr. Conventional transmission electron microscopy. Mol Biol Cell. 2014;25[3]:319-323. doi:10.1091/mbc.E12-12-0863)

Day(s) Performed

Monday through Friday

Seattle Children's Hospital Note:

TAT: 12 days 

Report Available

10 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

85390

88348

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PTEM Platelet TEM, B 79768-8

 

Result ID Test Result Name Result LOINC Value
CK109 Platelet TEM 79768-8
CK110 Interpretation 59466-3

Forms

1. Platelet Esoteric Testing Patient Information is required.

2. If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.