Sign in →

Test Code Proinsulin Proinsulin, Plasma

Reporting Name

Proinsulin, P

Useful For

As part of the diagnostic workup of suspected insulinoma


As part of the diagnostic workup of patients with suspected prohormone convertases1/3 deficiency


As part of the diagnostic workup of patients with suspected proinsulin mutations

Specimen Required

Patient Preparation:

1. Patient should be fasting for 8 hours.

2. Infants under 2 years of age should fast a maximum of 6 hours.

Collection Container/Tube: Ice-cooled, lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: After draw, chill the whole blood on ice for at least 10 minutes, then spin down in a refrigerated centrifuge.

Seattle Children's Hospital Note:

Collect 3.0 mL whole blood in an ice-cooled Lavender top.

Specimen Type

Plasma EDTA

Specimen Minimum Volume

0.25 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma EDTA Frozen 90 days

Day(s) and Time(s) Performed

Tuesday, Friday; 8 a.m.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Electrochemiluminescent Assay

Method Description

Sequential 2-site electrochemiluminescent immunoassay performed on the MesoScale Discovery instrument. The assay uses a monoclonal biotinylated anti-insulin capture antibody and an anti-c-peptide detection antibody labeled with SULFO-TAG. The signal is directly proportional to the amount of proinsulin in the sample. Assay calibration is traceable to the WHO 1st International Standard for human Proinsulin NIBSC code: 09/296. This assay demonstrates no cross-reactivity with insulin or C-peptide.(Unpublished Mayo method)

Reference Values

3.6-22 pmol/L

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
PINS Proinsulin, P 27882-0


Result ID Test Result Name Result LOINC Value
80908 Proinsulin, P 27882-0

Reject Due To


Mild OK; Gross reject


Mild OK; Gross OK


Mild OK; Gross OK