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Test Code Prot S Fr Protein S Antigen, Free

Clinical System Name

Protein S Antigen, Free

Synonyms

Protein S AG-Function

Protein S Level

Fractionated Protein S

PSAGF

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (3) 1.8 mL or (1) 2.7 mL

Minimum Vol: (2) 1.8 mL

 

Note: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended.

 

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill.

 

Specimen should be centrifuged within ONE hour of collection.

Processing Instructions

Note: Deliver blood to Core Coag bench. Coag Technologist will process. Specimen should be centrifuged within ONE hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube and make two aliquots of 0.6 mL, affix large Cerner label and store in the Core 6 (-70) freezer, Send Out rack.

 

Off-site Collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.

 

Specimen should be centrifuged within ONE hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label. Freeze two aliquots of 0.6 mL plasma in plastic tubes at -70C.

 

Ship frozen on dry ice.

Stability

Specimen Type Temperature Time
Citrated Platelet-Poor Plasma Room temp Unacceptable
  Refrigerated Unacceptable
  Frozen Y

 

Availability

STAT Performed TAT
N M, Th 1 - 8 d

 

Performing Laboratory

Harborview Medical Center

Clinical Coagulation Lab

325 9th Ave

Room GWH 47

Seattle, WA 98195

 

Phone Number: (206) 744-3128

Department

Department: Send Outs

Phone Number: (206) 987-2563

 

CPT Codes

85306

Methodology

Method: Optical, Stago STA LIA Free Protein S Antigen Assay

Analytical Volume: 0.5 mL Citrate Plasma

Limitations:

Reference Range

Female Male
Age % Range Age % Range
0 - 3 months 15 - 150 0 - 3 months 15 - 150
3 - 6 months 35 - 150 3 - 6 months 35 - 150
6 mos - 1 y 47 - 150 3 months 47 - 150
1 yr - 6 y 49 - 150 1 y - 6 y 49 - 150
6 y - 10 y 58 - 150 6 y - 10 y 58 - 150
10y - 55 - 150 10y - 65 - 150

 

Note: Elevated Protein S is not associated with thrombosis or bleeding.
 

Send Out Instructions

Reference Test Name: Protein S Antigen (Free)
Reference Test Number: PSAGF
Instructions: Send out Monday through Friday with the UW/HMC courier.