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Test Code Q FEVER Q Fever Antibody, IgG and IgM, Serum

Reporting Name

Q Fever Ab, IgG and IgM, S

Useful For

Diagnosing Q fever


Specimen Required


Container/Tube: 

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL


Specimen Type

Serum

Specimen Minimum Volume

0.25 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 7 days
  Frozen  7 days

Day(s) and Time(s) Performed

Monday through Friday; 9 a.m.

Seattle Children's Hospital Note:

TAT: 3 - 5 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Indirect Immunofluorescence

Method Description

An indirect immunofluorescence test is used for the measurement of IgM and/or IgG antibodies to Coxiella burnetii. Specific antibodies present in the serum of the patient react with rickettsial cells that have been previously fixed on a glass microscope slide. Fluorescein-labeled antihuman IgG or IgM conjugate is used to stain specific antibody bound to the substrate cells. The slides are examined with a fluorescence microscope for characteristic, apple-green fluorescence of the infected cell.(Edligner B: Immunofluorescence serology: a tool for prognosis of Q fever Diagn Microbiol Infect Dis 1985;3:343-351; package inserts: Q fever IFA IgG Focus Diagnostics, Inc., Cypress, CA; Q fever IFA IgM, Focus Diagnostics, Inc., Cypress, CA)

Reference Values

Q FEVER PHASE I ANTIBODY, IgG

<1:16

 

Q FEVER PHASE II ANTIBODY, IgG

<1:16

 

Q FEVER PHASE I ANTIBODY, IgM

<1:16

 

Q FEVER PHASE II ANTIBODY, IgM

<1:16

 

Reference values apply to all ages.

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86638 x 4

LOINC Code Information

Test ID Test Order Name Order LOINC Value
QFP Q Fever Ab, IgG and IgM, S 77175-8

 

Result ID Test Result Name Result LOINC Value
80965 Q Fever Phase I Ab, IgG 34716-1
24011 Q Fever Phase II Ab, IgG 34717-9
81115 Q Fever Phase I Ab, IgM 9710-5
24009 Q Fever Phase II Ab, IgM 9711-3
24010 Interpretation 69048-7

Testing Algorithm

See Infective Endocarditis: Diagnostic Testing for Identification of Microbiological Etiology in Special Instructions.

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

NA

Other

NA