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Test Code RMSF Spotted Fever Group Antibody, IgG and IgM, Serum

Reporting Name

Spotted Fever Group Ab, IgG, IgM, S
Seattle Children's Hospital Note:

SCH Clinical System Name: Rocky Mountain Spotted Fever Group Ab

Useful For

Aiding in the diagnosis of spotted fever group rickettsial infections


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Seattle Children's Hospital Note:

Collect 1.0 mL whole blood in a Red top.

Specimen Type

Serum

Specimen Minimum Volume

0.2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Day(s) Performed

Monday through Friday

Seattle Children's Hospital Note:

TAT: 3 - 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Method Name

Immunofluorescence

Method Description

Substrate slides containing antigen wells for measuring antibodies to both groups of Rickettsia (spotted fever and typhus) are obtained. The indirect immunofluorescence assay is a 2-stage "sandwich" procedure. In the first stage, the patient serum is diluted in yolk sac diluent. The diluted serum is placed on the slide in contact with the substrate and incubated. Following incubation, the slide is washed in buffered saline, which removes unbound serum antibodies. In the second stage, each antigen well is overlaid with fluorescein-labeled antibody to human IgG or IgM. The slide is incubated allowing antigen-antibody complexes to react with the fluorescein-labeled antihuman IgG or IgM. After the slide is washed, dried, and mounted, it is examined using fluorescence microscopy. Positive reactions appear as rickettsial bodies exhibiting bright apple-green cytoplasmic fluorescence against a background of orange to red yolk sac matrix. Semiquantitative end point titers are obtained by testing serial dilutions of positive specimens.(Package inserts: Rickettsia IFA IgM. DiaSorin Molecular LLC; 05/2018; Rickettsia IFA IgG. DiaSorin Molecular LLC; 05/2018)

Reference Values

IgG: <1:64

IgM: <1:64

Reference values apply to all ages.

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86757 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SFGP Spotted Fever Group Ab, IgG, IgM, S 90260-1

 

Result ID Test Result Name Result LOINC Value
84342 Spotted Fever Group Ab, IgG, S In Process
84346 Spotted Fever Group Ab, IgM, S In Process

Testing Algorithm

For more information see Acute Tick-Borne Disease Testing Algorithm.

Report Available

Same day/1 to 3 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.