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Test Code SEROTON B Serotonin, Serum

Reporting Name

Serotonin, S

Useful For

In conjunction with, or as an alternative to first-order test in the differential diagnosis of isolated symptoms suggestive of carcinoid syndrome, in particular flushing (5-HIAA or serum chromogranin A measurements are first-line tests)

 

Second-order test in the follow-up of patients with known or treated carcinoid tumors in serum specimens


Additional Testing Requirements


First-line testing for the diagnosis of carcinoid tumors with symptoms suggestive of carcinoid syndrome consists of urinary serotonin (SERU / Serotonin, 24 Hour, Urine), urinary 5-HIAA (HIAA / 5-Hydroxyindoleacetic Acid [5-HIAA], 24 Hour, Urine), and serum chromogranin A (CGAK / Chromogranin A, Serum).



Specimen Required


Patient Preparation: Patient should be off of medications that may affect serotonin concentrations including lithium, monoamine oxidase inhibitors, methyldopa, morphine, and reserpine.

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 2.5 mL

Collection Instructions: Centrifuge as soon as blood has clotted and aliquot into plastic vial.


Specimen Type

Serum

Specimen Minimum Volume

1.1 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 21 days
  Frozen  90 days
  Ambient  4 days

Day(s) and Time(s) Performed

Monday, Wednesday, Friday; 10 a.m.

Seattle Children's Hospital Note:

TAT: 6 - 8 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Method Description

Serotonin is removed from serum using reversed-phase solid-phase extraction (SPE). Separation is completed using a Bond Elut C18 SPE cartridge, and is eluted with 40% acetonitrile/1 mM ammonium acetate/0.1% formic acid. The eluate is analyzed using liquid chromatography/tandem mass spectrometry (LC-MS/MS) and quantified using a stable isotope-labeled internal standard, d(4)-serotonin.(Carling RS, Degg TS, Allen KR, et al: valuation of whole blood serotonin and plasma and urine 5-hydroxyindole acetic acid in diagnosis of carcinoid disease. Ann Clin Biochem 2002;39:577-582)

Reference Values

≤230 ng/mL

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

84260

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SER Serotonin, S 27057-9

 

Result ID Test Result Name Result LOINC Value
84395 Serotonin, S 27057-9

Reject Due To

Hemolysis

Mild OK; Gross OK

Lipemia

Mild OK; Gross OK

Icterus

Mild OK; Gross OK

Other

NA