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HelpTest Code TOBRA, TOBRA TR, TOBRA PK Tobramycin - Non-Cystic Fibrosis Patients
Clinical System Name
Tobramycin Level (Random, Peak, Trough)
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Green/Mint Top Lithium Heparin, Lt. Green/Lithium Heparin Microtainer, Gold SST, Gold Microtainer, Dark Green/Sodium Heparin, Red, Lavender/EDTA, Lavender/EDTA Microtainer
Preferred Vol: 1.0 mL
Minimum Vol: 0.5 mL
Note: Every 8 hour dosing: Infuse IV over 30 minutes. Draw peak level after 3rd or 4th dose 30 minutes after the end of the IV infusion. Trough levels should be drawn 30 minutes before the 4th dose. Document the date and time of infusion and collections. Also, document whether the sample is a peak, a trough, or a random level.
Processing Instructions
Reject due to: Insufficient quantity, Age of specimen
Spin: Y
Aliquot: Y
Storage location: Core Chemistry Refrigerator
Off-site collection: Every 8 hour dosing: Infuse IV over 30 minutes. Draw peak level after 3rd or 4th dose 30 minutes after the end of the IV infusion. Trough levels should be drawn 30 minutes before the 4th dose. Document the date and time of infusion and collections. Also, document whether the sample is a peak, a trough, or a random level. Specimen should be centrifuged within 1 hour of collection. Spin blood and transfer serum or plasma to plastic tube and refrigerate or freeze.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum or Plasma | RT |
≤ 2 h |
| Serum or Plasma | 2-4 C |
≤ 7 d |
| Serum or Plasma | -20 C or -70 C |
≤ 14 d |
Availability
| STAT | Performed | TAT |
|---|---|---|
| Y | 24/7 | 1 h |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Core Chemistry
Phone Number: 206-987-2617 (Client Services)
CPT Codes
80200
Methodology
Method: Two Point Rate method by Vitros 4600
Analytical Volume: 0.06 mL + 0.03 mL dead space
Reference Range
| mcg/mL | |
| Peak | 4 - 12 |
| Trough | 0 - 2 |
| Toxic | >12 |
Critical Values
> 12 mcg/mL