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Test Code U Beta 2 Micro Beta-2 Microglobulin (B2M), Urine

Specimen Required

Supplies: Urine Tubes, 5 mL

Container/Tube: Plastic, urine tube

Specimen Volume: 3 mL

Collection Instructions:

1. Patient should empty bladder.

2. Have patient drink at least 0.5 liters of water.

3. Within 1 hour, collect a random urine specimen.

4. Add 1 M NaOH as preservative to the collection. This preservative is intended to achieve a pH of between approximately 6 and 8.

Seattle Children's Hospital Note:

Children's Regional Clinic Collection: Send sample refrigerated to SCH Main Lab for pH adjustment.

Useful For

Evaluation of renal tubular damage


Monitoring exposure to cadmium and mercury

Method Name

Automated Chemiluminescent Immunometric Assay

Reporting Name

Beta-2 Microglobulin, U
Seattle Children's Hospital Note:

Clinical System Name:  Beta 2 Microglobulin, Urine

Specimen Type


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Urine Frozen (preferred) 14 days
  Refrigerated  48 hours

Reject Due To









Reference Values

≤300 mcg/L

Method Description

Testing is performed on the Immulite 2000. Immulite 2000 Beta-2 Microglobulin is a solid phase, 2-site chemiluminescent enzyme-labeled immunometric assay. The solid-phase bead is coated with an affinity-purified murine monoclonal anti-beta-2 antibody. The serum sample and alkaline phosphatase conjugated affinity-purified goat polyclonal anti-beta-2 antibody are incubated to bind beta-2 microglobulin into an antibody sandwich complex.


The chemiluminescent substrate, a phosphate ester of adamantyl dioxetane, in the presence of alkaline phosphatase produces light proportional to the concentration of the beta-2 microglobulin in the sample.(Product Information: IMMULITE 2000 Beta-2 Microglobulin, Siemens Healthcare Diagnostics, 2012-11-05)

Day(s) and Time(s) Performed

Monday, Wednesday, Friday

Seattle Children's Hospital Note:

TAT: 3 - 5 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
B2MU Beta-2 Microglobulin, U 1953-9


Result ID Test Result Name Result LOINC Value
B2MU Beta-2 Microglobulin, U 1953-9