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Test Code U Beta 2 Micro Beta-2 Microglobulin, Random, Urine


Specimen Required


Patient Preparation: For 12 hours before specimen collection, do not take multivitamins or dietary supplements containing biotin (vitamin B7) which is commonly found in hair, skin, and nail supplements and multivitamins.

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Container/Tube: Plastic, urine tube

Specimen Volume: 1.0 mL

Collection Instructions:

1. Patient should empty bladder.

2. Have patient drink at least 0.5 liters of water.

3. Within 1 hour, collect a random urine specimen.

4. Add 1 M sodium hydroxide (NaOH) as preservative to the collection. This preservative is intended to achieve an approximate pH of between 6 and 8.


Seattle Children's Hospital Note:

Children's Regional Clinic Collection: Send sample refrigerated to SCH Main Lab for pH adjustment.

Useful For

Evaluation of renal tubular damage

 

Monitoring exposure to cadmium and mercury

Method Name

Automated Chemiluminescent Immunometric Assay

Reporting Name

Beta-2 Microglobulin, U
Seattle Children's Hospital Note:

Clinical System Name:  Beta 2 Microglobulin, Urine

Specimen Type

Urine

Specimen Minimum Volume

0.5mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Frozen (preferred) 14 days
  Refrigerated  48 hours

Reject Due To

Specimen with pH <6 Reject

Reference Values

≤300 mcg/L

Method Description

Testing is performed on the Immulite 2000. The Immulite 2000 Beta-2 Microglobulin assay is a solid phase, 2-site chemiluminescent enzyme-labeled immunometric assay. The solid-phase bead is coated with an affinity-purified murine monoclonal anti-beta-2 antibody. The serum sample and alkaline phosphatase conjugated affinity-purified goat polyclonal anti-beta-2 antibody are incubated to bind beta-2 microglobulin into an antibody sandwich complex.

 

The chemiluminescent substrate, a phosphate ester of adamantyl dioxetane, in the presence of alkaline phosphatase produces light proportional to the concentration of the beta-2 microglobulin in the sample.(Package insert: IMMULITE 2000 Beta-2 Microglobulin. Siemens Healthcare Diagnostics; 03/15/2018)

Day(s) Performed

Monday, Wednesday, Friday

Seattle Children's Hospital Note:

TAT: 3 - 5 days

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

82232

LOINC Code Information

Test ID Test Order Name Order LOINC Value
B2MU Beta-2 Microglobulin, U 1953-9

 

Result ID Test Result Name Result LOINC Value
B2MU Beta-2 Microglobulin, U 1953-9

Forms

If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.