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Test Code VANC, VANC TR, VANC PK Vancomycin

Important Note

No add-ons after 8 hours of collection if specimen is still on gel separator.

Clinical System Name

Vancomycin (Random, Peak, Trough)

Sample Requirements

Specimen:  Whole Blood

Container(s): Lt. Green/Mint Top Lithium Heparin, Lt. Green/Lithium Heparin Microtainer, Gold SST, Gold Microtainer, Dark Green/Sodium Heparin, Red

Preferred Vol:  1.0 mL

Minimum Vol:  0.5 mL

Note:  Draw trough level ½ hour before the start of an infusion. Note whether specimen is peak, trough, or random. As of January 2004, vancomycin peak monitoring is no longer recommended, per Pharmacy and Therapeutics Committee and Infectious Diseases. If drawing a peak, draw at one hour after the end of a 1 hour infusion.

Processing Instructions

Reject due to: Age of specimen, incorrect collection container

Spin: Y

Aliquot:  Y

Storage location: Core 5 Refrigerator

 

Off-site collection: Draw trough level ½ hour before the start of an infusion. Note whether specimen is peak, trough, or random. As of January 2004, vancomycin peak monitoring is no longer recommended, per Pharmacy and Therapeutics Committee and Infectious Diseases. If drawing a peak, draw at one hour after the end of a 1 hour infusion. Specimen should be centrifuged within 2 hours of collection. Spin blood and transfer serum or plasma to plastic tube and refrigerate or freeze.

Stability

Specimen Type Temperature Time
Serum or Plasma RT

≤ 2 h
Serum or Plasma 2-8 C

≤ 3 d
Serum or Plasma -20 C or -70 C

≤ 14 d

Availability

STAT Performed TAT
Y 24/7 1 h

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Core Chemistry

Phone Number: 206-987-2617 (Client Services)

CPT Codes

80202

Methodology

Method: Two Point Rate by Vitros 4600

Analytical Volume:  0.06 mL + 0.03 mL dead space

Reference Range

Peak:

20 - 40 µg/mL

Trough:  

8 - 15 µg/mL

Toxic:

>40 µg/mL

 

Critical Values

> 40.0 µg/mL