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Test Code VIT C Ascorbic Acid (Vitamin C), Plasma

Useful For

Diagnosing vitamin C deficiency


As an aid to deter excessive intake

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Ascorbic Acid, P
Seattle Children's Hospital Note:

Clinical System Name: Vitamin C Level

Specimen Type

Plasma Heparin
Seattle Children's Hospital Note:

Note: Protect from light by wrapping in paper towel and securing with one piece of tape. Avoid hemolysis.

Specimen Required

Patient Preparation: Fasting overnight (12-14 hours) (infants-draw prior to next feeding). Water can be taken as needed.

Supplies: Amber Frosted Tube, 5 mL (T192)

Collection Container/Tube: Green top (heparin)

Submission Container/Tube: Amber vial (T192)

Specimen Volume: 1 mL

Collection Instructions

1. Immediately place specimen on wet ice. Maintain specimen on wet ice and process within 4 hours of draw.

2. Centrifuge at 4° C, aliquot plasma into amber vial to protect from light and freeze immediately on dry ice.

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma Heparin Frozen 14 days

Reject Due To


Mild Reject; Gross reject


Mild OK; Gross OK


Mild OK; Gross OK


Specimen not protected from light, EDTA plasma.

Reference Values

0.4-2.0 mg/dL

Method Description

Samples are diluted and extracted online extraction by high-turbulence liquid chromatography, with detection by tandem mass spectrometry.(Unpublished Mayo Method)

Day(s) and Time(s) Performed

Tuesday, Thursday, Friday; Evening

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
VITC Ascorbic Acid, P 1903-4


Result ID Test Result Name Result LOINC Value
8312 Ascorbic Acid, P 1903-4