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Test Code LAB2005 NT-pro B-Type Natriuretic Peptide

Important Note

BNP testing will be discontinued August 2026, and replaced with NT-proBNP. In the intervening time, a new panel (LAB3939) will measure NT-proBNP at the same time as BNP to facilitate re-baselining patients with the new test, NT-proBNP. 

Clinical System Name

NT-pro B-Type Natriuretic Peptide

Synonyms

NT-proBNP

 

Sample Requirements

Specimen: Whole Blood

Container: Lt Green/Mint top - Lithium Heparin, Lt Green LiHep Microtainer, Gold SST, Gold Microtrainer, Red top - Plain

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL Microtainer, 1.0 mL Macrotainer

Note: Microtainers used for capillary collections are unacceptable.

Processing Instructions

Reject due to: Insufficient Quantity, Capillary collections, Gross Hemolysis, Age of Specimen, Specimens drawn in glass tubes.

Spin: Y

Aliquot: Y

Storage location: Core 5 Chemistry Refrigerator

Off-site colleciton: Specimen should be centrifuged within 2 hours of collection. Spin blood and transfer plasma to plastic tube and refrigerate. Method affected by prolonged exposure to RBCs.

Stability

Specimen Type Temperature  Time
Plasma or Serum RT 24 h
Plasma or Serum 2 - 8 C 48 h
Plasma or Serum -20 C 1 w

 

Availability

STAT Performed TAT
Y 24/7 2 h

 

Performing Laboratory

Seattle Children's Laboratory

Department

Department: Core Chemistry

Phone Number: (206) 987-2617 (Client Services)

Methodology

Method: Chemiluminescent micropartical immunoassay (CMIA) by Abbott Alinity i

Analytical volume: 150 uL

Limitiations: Elevated NT-proBNP concentrations may be associated with impaired renal function (estimated glomerular filtration rate [eGFR] < 60 mL/min/1.73 m2).

Reference Range

Age pg/mL
< 1 < 1785
≥ 1 y to 18 y < 270
> 18 y < 300

 

CPT Code

83880

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