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Test Code LAB2396 Chlamydia trachomatis PCR

Important Note

For sexual assault cases and urgent / critical cases, please contact the Micro lab directly at 7-2585

Infectious

Synonyms

Chlamydia PCR

Chlamydia trachomatis amplified probe

LAB2396

Sample Requirements

Specimen: Dirty Catch Urine*, Endocervical, Vaginal, Throat, Rectal

 

NOTE: For non-FDA approved specimens (Eye, Urethral, Penile), order a Miscellaneous Lab Test.  Collect samples using the Apitma Unisex or Aptima Multitest Collection Kit. Eye samples will be sent to Mayo Clinic Laboratory for testing.  Penile and Urethral samples will be sent to UWMC Laboratory for testing.

 

Container:

  • Sterile Container for all Urines
  • "GeneXpert Vaginal Endocervical Collection kit" CT/NG swabs for all in house PCR testing
  • If sample is bloody collect instead:
    • "Aptima Unisex Collection kit" for Endocervical or Urethral sites.
    • "Aptima Multitest Collection kit" for Vaginal Penile, Throat, or Rectal Sites

Preferred Vol: 10mL for Urine

Minimum Vol: 7mL for Urine**

Please consult the Photo Swab Guide to determine which swab to collect.

 

*Dirty Catch urine is the first stream of the first morning void (for maximum sensitivity), or the first stream at least 1 hour after the last void. Patient should not perform any genital cleaning before collection. If the patient has submitted a clean catch sample, a dirty catch sample can be submitted at least one hour since the last void. Clean catch urines are unacceptable for testing and will be REJECTED.

 

**Urine samples with suboptimal volumes (between 2mL - 7mL) will be sent to the University of Washington for testing.  Send urine to lab in sterile container.  Urine samples of less than 2mL are unacceptable and will be REJECTED.

 

Bloody swabs/urine samples will be sent to University of Washington for testing.  Send bloody urine to lab in sterile container.  Collect bloody Vaginal or Endocervical samples with Gen-Probe APTIMA collection kit. Call Microbiology (206-987-2585) for APTIMA Kits.

Samples received in Aptima collection kits will be forwarded to the University of Washington for testing.

 

Processing Instructions

CPA: Send sample to Microbiology for processing.

Regional Clinic collection: Send to SCH Main Campus for testing. Refrigerate before and during courier transport. If urine sample received is less than minimal volume, call Microbiology at 206-987-2585 for consult on running or cancelling the test. Additional questions on collection and other specimen sites, contact CF/ Molecular Microbiology Supervisor or Director of Microbiology.

 

Stability

Urine
Temp Time
Room temp 24 hours
Refrigerated 1 week
Frozen N

 

Xpert Vaginal/Endocervical Swabs
Temp Time
Room temp 60 days
Refrigerated 60 days
Frozen N

 

Aptima Multitest Swab Specimen Collection Kit
Temp Time
Room temp 30 days
Refrigerated 30 days
Frozen N

 

Aptima Unisex Swab Specimen Collection Kit
Temp Time
Room temp 30 days
Refrigerated 30 days
Frozen N

 

Availability

Procedure Performed Turn Around Time (TAT)
In-House PCR (Cepheid) 24/7 3 hours
APTIMA PCR (for bloody samples sent to UW for testing) 24/7

3-5 days

 

Performing Laboratory

Seattle Children's Laboratory

 

Department

Microbiology Laboratory

CPT Codes

87491

Description

This includes nucleic acid amplification for Chlamydia trachomatis with amplified probe technique.

For forensic evaluation of Chlamydia trachomatis, please see Sexual Assault Photo Swab Guide and order CT GC PCR through the Sexual Assault PowerPlan.

Clincal System Name

Chlamydia trachomatis PCR

Reporting Information

Final Report (Cepheid): 3 hours

Final Report (Aptima): 3-5 days

Notifiable Result

See Link below for Notifiable Conditions for WA state labs:

https://doh.wa.gov/public-health-healthcare-providers/notifiable-conditions

 

See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:

https://www.doh.wa.gov/Portals/1/Documents/1200/LHJCommunicableDiseaseReporting.pdf